The tumor suppressor Scrib interacts with the zyxin-related protein LPP, which shuttles between cell adhesion sites and the nucleus
© Petit et al; licensee BioMed Central Ltd. 2005
Received: 10 September 2004
Accepted: 13 January 2005
Published: 13 January 2005
At sites of cell adhesion, proteins exist that not only perform structural tasks but also have a signaling function. Previously, we found that the Lipoma Preferred Partner (LPP) protein is localized at sites of cell adhesion such as focal adhesions and cell-cell contacts, and shuttles to the nucleus where it has transcriptional activation capacity. LPP is a member of the zyxin family of proteins, which contains five members: ajuba, LIMD1, LPP, TRIP6 and zyxin. LPP has three LIM domains (zinc-finger protein interaction domains) at its carboxy-terminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains.
To catch the role of LPP at sites of cell adhesion, we made an effort to identify binding partners of LPP. We found the tumor suppressor protein Scrib, which is a component of cell-cell contacts, as interaction partner of LPP. Human Scrib, which is a functional homologue of Drosophila scribble, is a member of the leucine-rich repeat and PDZ (LAP) family of proteins that is involved in the regulation of cell adhesion, cell shape and polarity. In addition, Scrib displays tumor suppressor activity. The binding between Scrib and LPP is mediated by the PDZ domains of Scrib and the carboxy-terminus of LPP. Both proteins localize in cell-cell contacts. Whereas LPP is also localized in focal adhesions and in the nucleus, Scrib could not be detected at these locations in MDCKII and CV-1 cells. Furthermore, our investigations indicate that Scrib is dispensable for targeting LPP to focal adhesions and to cell-cell contacts, and that LPP is not necessary for localizing Scrib in cell-cell contacts. We show that all four PDZ domains of Scrib are dispensable for localizing this protein in cell-cell contacts.
Here, we identified an interaction between one of zyxin's family members, LPP, and the tumor suppressor protein Scrib. Both proteins localize in cell-cell contacts. This interaction links Scrib to a communication pathway between cell-cell contacts and the nucleus, and implicates LPP in Scrib-associated functions.
At the heart of structural and functional integrity of multicellular entities is the ability of each and every cell of it to successfully integrate signals arising from soluble factors, cell-substratum adhesion and cell-cell adhesion . Correct processing of these signals allows appropriate cellular growth, differentiation, and tissue morphogenesis, but malfunctions often lie at the basis of pathologies such as tumor growth and metastasis. At sites of cell adhesion, more and more proteins are being identified that not only play a role in maintaining cell shape and motility but that, in addition to these structural functions, are also implicated in signaling events. Because of this dual function, these proteins have to interact, via multiple binding motifs, with components of both the actin cytoskeleton and signaling pathways that regulate e.g. gene expression.
Originally, we identified the LPP gene, as being the preferred translocation partner of the HMGA2 gene in a subgroup of lipomas, which are benign tumors of adipose tissue . In these tumors, HMGA2/LPP fusion transcripts are expressed and identical fusion transcripts have also been found in a subgroup of pulmonary chondroid hamartomas , in a parosteal lipoma , and in a soft tissue chondroma . In a case of acute monoblastic leukemia, the LPP gene acts as translocation partner of the MLL gene and the tumor expresses MLL/LPP fusion transcripts . All tumor-specific fusion transcripts that are expressed in the above mentioned tumors encode similar LPP fusion proteins containing AT-hooks (DNA binding domains) of the HMGA2 or MLL proteins followed by LIM domains of LPP. These fusion proteins are mainly expressed in the nucleus .
At cell adhesions, the LPP protein interacts with α-actinin and VASP (vasodilator-stimulated phosphoprotein) via its pre-LIM region that contains an α-actinin binding site located near its N-terminus and two VASP-binding ("FP4")-motifs (Fig. 1A) [11, 12]. In the nucleus, LPP has transcriptional activation capacity in reporter gene assays suggesting that it is involved in the regulation of gene expression . The nucleocytoplasmic distribution of this protein involves a nuclear export signal (NES) that also resides in the pre-LIM region (Fig. 1A) . Recently, we have shown that the LIM domains of LPP cooperate to target the protein to focal adhesions, and that the linker between LIM domains 1 and 2 plays a pivotal role in this targeting . When overexpressed in the cytoplasm of cells, these LIM domains deplete endogenous LPP and vinculin from focal adhesions suggesting a role for LPP in focal adhesion assembly . Recently, LPP was found to be highly expressed in smooth muscle [14, 15], and a role for LPP in regulating cell motility was proposed .
In an effort to learn more about the molecular function of LPP, we performed a yeast two-hybrid screening experiment to identify potential LPP-interacting proteins. Here, we report that LPP interacts with Scrib, a member of the LAP (leucine-rich repeat and PDZ domain) family of proteins . Scrib is a functional homologue  of Drosophila Scribble, a tumor suppressor that plays a role in the regulation of cellular adhesion, cell shape and polarity [18, 19]. In follow-up of the results of the yeast two-hybrid screening, we have performed various experiments to find out whether the observed interaction also occurs in mammalian cells and have substantiated this interaction in vitro and in vivo. Furthermore, we have studied whether or not the Scrib protein plays a role in the subcellular targeting of LPP.
Screening for LPP-interacting proteins by yeast two-hybrid
In a previous study , we showed that the LIM domains of LPP are the major units for targeting LPP to focal adhesions. LIM domains are cysteine- and histidine-rich domains that form two zinc fingers capable of mediating protein-protein interactions [20, 21]. However, the protein(s) that is/are responsible for the targeting of LPP to focal adhesions, i.e. protein(s) that bind(s) to the LIM domains of LPP, are not yet known. To identify protein binding partners of the LIM domains of LPP, we performed a yeast two-hybrid screening experiment. We made use of a yeast two-hybrid system that is based on transcriptional activation of two reporter genes HIS3 and LacZ whose expression is driven by upstream GAL4 DNA-binding sites. Because all three LIM domains of LPP cooperate to target LPP to focal adhesions , we initially focused on a screening using a bait that contained all three LIM domains. Unlike in mammalian cells, where we have shown that the three LIM domains of LPP have transcriptional activation capacity , this bait, although well expressed, did not activate the reporter genes in yeast cells (results not shown). This is similar to what has been found for zyxin's LIM domains , but in contrast to what has been found for the three LIM domains of TRIP6 that do activate reporter genes in yeast . However, the bait containing all three LIM domains of LPP appeared to be very sticky since thousands of yeast colonies were obtained in which both reporter genes were activated. In an effort to reduce background activity, we deleted the first LIM domain, or the first and the second LIM domain, in the bait, leaving the two most carboxy-terminal, or the most carboxy-terminal LIM domain(s) intact, respectively. These deletions completely abolished all background activity making these baits the baits of choice to perform a library screening. Here, we report about the screening that was performed with the bait containing only the most carboxy-terminal LIM domain of LPP. As described before , we showed that the third LIM domain of LPP only has a very weak targeting capacity for focal adhesions. This makes it very unlikely that, by using this bait, we would pick up a protein that targets LPP to these structures, which was the initial goal of our studies. Indeed, our screening did not reveal any focal adhesion binding partners of LPP, however, in stead, we found another very interesting LPP-interacting protein as will be outlined in the following sections.
A mouse embryonal cDNA library was screened using a bait (pGBT9-LPPWT) containing the third LIM domain and carboxy-terminus of human LPP (amino acids 531–612). Among ~1.0 × 106 yeast cotransformants (Leu+ and Trp+), 56 clones were His+ of which 23 were LacZ+ too. PCR analysis of these His+/LacZ+ clones, using prey-specific insert-flanking primers, revealed that 21 of the 23 obtained clones, contained a prey-construct having a 2 kb cDNA insert (results not shown). Subsequent fragmentation of the obtained 2 kb PCR products, representing the cDNA inserts of the prey-constructs, using the HaeIII restriction enzyme (frequent cutter), indicated that all 21 isolated prey-constructs, having a 2 kb insert, were identical. The 2 kb cDNA insert of one representative prey-construct was completely sequenced and the sequence was submitted to the NCBI database (Genbank accession no. AF271735).
A BLAST (Basic Local Alignment Search Tool)-search revealed that this mouse prey-construct encoded an amino- and carboxy-terminally truncated protein comprising four PDZ domains that was almost identical to the human Scrib protein (Fig. 1B), indicating that the prey-construct represented mouse Scrib. The Scrib protein contains a set of 16 leucine-rich repeats (LRRs) near its amino-terminus and four PDZ (PSD-95, Discs large, ZO-1) domains distributed throughout the remainder of the protein (Fig. 1B). The partial mouse Scrib protein, expressed by the prey-construct, corresponded to amino acids 709 – 1242 of human Scrib (Fig. 1B).
Interaction of LPP with Scrib in the yeast two-hybrid system
LPP binds to the PDZ domains of Scrib via its C-terminal tail
Since the pACT2-mScrib prey-construct contained four PDZ domains, and since PDZ domains are one of the most commonly found protein-protein interaction domains in organisms from bacteria to humans , it was most likely that Scrib would bind to LPP via its PDZ domains. The LPP-bait that was used to screen the library was pGBT9-LPPWT containing the third LIM domain and carboxy-terminus of human LPP. Although PDZ domains have been shown to bind LIM domains , binding to carboxy-terminal peptides appears to be the typical mode of interaction . The common structure of PDZ domains comprises six β strands (βA-βF) and two α helices (αA and αB), which fold in an overall six-stranded β sandwich . The binding specificity of PDZ domains is critically determined by the interaction of the first residue of helix α B (position αB1) and the side chain of the -2 residue of the C-terminal ligand. This forms the basis for PDZ classification . Since all four PDZ domains of Scrib contain a histidine at position αB1, they are classified as class I PDZ domains. Therefore, based on what has been demonstrated for this subclass of PDZ domains [25, 26], the carboxy-terminal sequence of Scrib target proteins is predicted to require a hydrophobic amino acid (h) at the 0 (carboxy-terminus) position, and a serine (S) or threonine (T) at the -2 position.
Theoretically, the carboxy-terminus of the LPP protein, being -STDL, thus completely fulfils the criteria for binding to the PDZ domains of Scrib. To evaluate these predictions experimentally and to demonstrate that the binding of LPP to Scrib is specific, we performed yeast two-hybrid experiments using pGBT9-LPPWT as well as pGBT9-LPPS609A, pGBT9-LPPT610A, pGBT9-LPPD611A and pGBT9-LPPL612A as bait. The last four baits are identical to pGBT9-LPPWT except for a point mutation to alanine, respectively introduced at serine609 (-3 position), threonine610 (-2 position), aspartate611 (-1 position) and leucine612 (position 0). As prey, we used pACT2-mScrib. As summarized in Table 1, this alanine-scan mutant analysis identified threonine610 (-2 position) and leucine612 (0 position) of LPP as being essential for binding to Scrib indicating a PDZ domain-mediated specific interaction between Scrib and the carboxy-terminus of LPP.
Interaction of LPP with PDZ domains of proteins different from Scrib
LPP interacts with Scrib PDZ domains in mammalian cells
These results indicate that LPP binds to Scrib PDZ domains and that this binding is abolished when amino acids at position 0 or -2 are mutated.
Development and characterization of Scrib antibodies
Scrib is not localized in focal adhesions in CV-1 and MDCKII cells, and is dispensable for targeting LPP to these structures
As deduced from these results, we hypothesized that Scrib was not involved in targeting LPP to focal adhesions. Indeed, evidence for this hypothesis was obtained by transfecting CV-1 cells with a construct expressing GFP-LPPWT containing full length wild-type LPP, or GFP-LPPT610A, which is identical to GFP-LPPWT except for a point mutation to alanine introduced at threonine610, which abolishes binding to Scrib. No difference in focal adhesion localization could be detected between wild-type and mutated GFP-LPP fusion proteins (Fig. 4, lower panels).
Scrib and LPP are dispensable to target each other to cell-cell contacts
Since Scrib and LPP both localize in cell-cell contacts [11, 17] (Fig. 5, upper panels), we investigated whether Scrib was responsible for targeting LPP to cell-cell contacts. For this, we made stable MDCKII cell lines expressing wild-type and mutated GFP-coupled forms of the LPP protein, of which the mutant form is not able to bind anymore to Scrib. However, as shown in Fig. 5, lower panels, LPP proteins that could not bind to Scrib anymore were still able to localize in cell-cell contacts in a similar way as their wild-type counterparts. These results indicate that Scrib is not responsible for targeting LPP to cell-cell contacts.
We next investigated whether LPP was responsible for targeting Scrib to cell-cell contacts. To look into this aspect, we made stable MDCKII cell lines expressing either wild-type full length Scrib-GFP or a mutated Scrib-GFP protein lacking all four PDZ domains (deletion of amino acids 725–1227). However, both the full length Scrib-GFP protein as well as the mutated form lacking all four PDZ domains localized equally well in cell-cell contacts (Fig. 5, lower panels). These results indicate that the PDZ domains of Scrib are dispensable for targeting the protein to cell-cell contacts, and as a consequence LPP is not necessary to locate Scrib in cell-cell contacts.
In summary, these results indicate that LPP and Scrib are dispensable to target each other to cell-cell contacts.
There is a direct interaction between the carboxy-terminus of LPP and the PDZ domains of Scrib
To further investigate the requirements in the Scrib protein for binding to LPP, we performed additional GST pull-down experiments. From our previously described experiments (yeast and mammalian two-hybrid), it was clear that the PDZ domains of Scrib bind to LPP. These findings were confirmed by using GST pull-down: as shown in Fig. 6C, upper panel, a portion of the Scrib protein encompassing all four PDZ domains was efficiently pulled down by GST-LPP-LTWT. To find out which of the four PDZ domains of Scrib was responsible for the observed interaction with LPP, we mutated the PDZ domains of Scrib, one at the time, by destroying their carboxylate binding loop (LG → AE), and tested how efficiently these mutated proteins were pulled down by GST-LPP-LTWT. From the results, which are presented in Fig. 6C, we can conclude that all four PDZ domains of Scrib more or less contribute to the binding to LPP, but that PDZ 3 is most important, since binding to GST-LPP-LTWT was almost completely abolished when the carboxylate binding loop of this PDZ domain was destroyed.
Scrib can target LPP to an ectopic location in vivo through its PDZ domains
To investigate the importance of the PDZ domains of Scrib in this recruitment of LPP, we deleted all four PDZ domains (amino acids 724–1192) from Xpress-hScrib-mito (=Xpress-hScribdPDZ-mito) and tested whether this PDZ-less protein still was able to recruit LPP to mitochondria. As shown in Fig. 7, lower panels, Xpress-hScribdPDZ-mito lost its ability to recruit LPP to mitochondria. These results indicate that Scrib can recruit LPP to an ectopic location in vivo, and that the PDZ domains of Scrib are an absolute requirement for this activity.
As mentioned above, the Xpress-hScrib-mito chimera localized to mitochondria in all cells that expressed this protein. However, LPP, which was co-expressed, was only recruited to mitochondria in a small fraction of these cells. This issue will be further addressed in the Discussion section.
In the course of our studies of chromosomal aberrations in benign tumors, we have previously discovered the LPP gene as being rearranged in certain subtypes of these tumors , and identified the LPP protein as a member of the zyxin family of proteins . In this study, we report that LPP specifically interacts with Scrib. We provide evidence that this interaction is mediated by the carboxy-terminus of LPP on the one hand, and the PDZ domains of Scrib on the other hand. Futhermore, we show that Scrib is not necessary for targeting LPP to focal adhesions, and that Scrib and LPP are dispensable to target each other to cell-cell contacts.
Scrib is a member of the LAP (LRR (leucine-rich repeat) and PDZ (PSD-95/Discs-large/ZO-1)) family of membrane-associated proteins that play a role in the regulation of cell polarity . LAP family members have been identified in mammals (Erbin, Densin-180, Lano, and Scrib) [29–32], in Caenorhabditis elegans (LET-413) , and in Drosophila melanogaster (Scribble) . LAP proteins contain a set of leucine-rich repeats (LRRs) at their amino-terminus, and either four (Scrib and Scribble), one (Erbin, Densin-180 and LET-413) or no (Lano) copies of the PDZ domain. A specific characteristic of these proteins are the LAP-specific domains (LAPSa and b), which are located carboxy-terminally of the LRRs .
Most information regarding the function of Scrib comes from studies in Drosophila melanogaster. Drosophila Scribble was identified as being required for the apical confinement of polarity determinants in epithelia . Mutations in Scribble cause aberrant cell shapes and loss of the monolayer organization in embryonic epithelia. Scribble is localized in septate junctions and loss of Scribble function results in the misdistribution of apical proteins and adherens junctions to the basolateral cell surface. Subsequent studies in Drosophila provided evidence that Scribble is a tumor suppressor and cooperates with two other tumor suppressors, Lethal giant larvae (Lgl) and Discs-large (Dlg) to regulate cell polarity and growth control . Recently, these three tumor suppressors were shown to regulate cell size and mitotic spindle asymmetry in Drosophila neuroblasts . The role of Scribble in tumorigenesis was further supported by the discovery that Scribble mutants cooperate with oncogenic Ras or Notch to cause neoplastic overgrowth of the eye disc , and that cooperation between oncogenic Ras and inactivation of Scribble leads to metastatic behavior . Additional studies in Drosophila implicate Scribble in the regulation of synaptic plasticity and synaptic vesicle dynamics [38, 39], and show that Scribble is essential for olfactory behavior in Drosophila .
As for mammalian Scrib, little information is available at the moment. Relating to the control of cell polarity and proliferation, human Scrib was found to be a functional homologue of the Drosophila scribble protein . Polarity defects and tumorous overgrowth of Scribble-mutant flies are rescued by human Scrib predicting an important role for human Scrib in the suppression of mammalian tumorigenesis. Further support for this hypothesis, was obtained by the fact that human and mouse Scrib are targeted for degradation by high-risk papillomavirus E6 proteins [32, 41]. Human papilloma viruses cause papillomas or warts on skin, genital tissues, and the upper respiratory tract, and high-grade lesions progress to carcinomas at a high frequency. The high-risk subgroup of human papilloma viruses detected in these lesions have been causally linked to the development of over 90% of uterine cervical carcinomas, the second leading cause of cancer-related deaths among women world-wide. High-risk papilloma virus E6 proteins direct Scrib for degradation by directly binding to the PDZ-domains of Scrib.
In this regard, it is noteworthy that we observed a remarkable aspect regarding the expression levels of Scrib in a number of mammalian cell lines. As already mentioned before (Fig. 3), we noticed that 293T cells expressed much lower levels of Scrib as compared to 293 cells. 293T cells are derived from 293 but, in contrast, these cells stably express Simian Virus 40 largeT antigen. SV40 large T is a powerful oncoprotein capable of transforming a variety of cell types . Its transforming activity is attributed to its binding and manipulation of the function of certain key tumor suppressors and cell regulatory proteins such as retinoblastoma and p53. However, certain factors that contribute to its full transformation potential are not yet completely understood. We hypothesize that large T induces the downregulation of Scrib expression, and that Scrib contributes to the transformation potential of SV40 large T.
In addition to its role as a tumor suppressor, Scrib was also implicated in the regulation of planar cell polarity, a role that is not established for Drosophila Scribble , and it was shown that disruption of Scrib is the causal factor for the severe neural tube defects that occur in the circletail mouse . Disruption of neural tube closure leads to a group of disorders termed neural tube defects, which are one of the commonest causes of congenital malformation and lethality in humans. The most severe form of neural tube defect is craniorachischisis, in which almost the entire brain and spinal cord remain open. Craniorachischisis comprises 10–20% of human neural tube defects, and is caused by a failure to initiate neural tube formation at the start of neurulation. Circletail is one of only two mouse mutants that exhibit craniorachischisis. The fact that Scrib was identified as the gene that was mutated in this mouse attributes an important role for Scrib in development .
We show here that Scrib is expressed equally well in very different cell types, such as Jurkat cells, which are human T lymphocytes, epithelial cells such as 293 and MDCKII cells, and in fibroblasts such as CV-1 cells. As described above, the function of Scrib and its Drosophila ancestor Scribble have been mainly addressed in epithelial cells. To our knowledge, nothing is known yet about the function of Scrib in other cell types such as lymphocytes and fibroblasts.
We show here that LPP specifically binds to and partially co-localizes with Scrib in cell-cell contacts of epithelial and fibroblastic cell lines. Previous studies have shown that PDZ domain proteins play an important role in the targeting of proteins to specific membrane compartments and in the assembly of these proteins into supramolecular complexes . Therefore, we investigated whether Scrib was essential to localize LPP in cell-cell contacts. However, as demonstrated by these experiments, Scrib is not necessary to target LPP to these structures. These findings are similar to what has been found for targeting of zyxin family members to focal adhesions. Recently, zyxin and TRIP6 were shown to interact with members of the p130Cas family of signal transducers, which are focal adhesion components . This interaction is primarily mediated by the LIM domains of zyxin and TRIP6. One specific function associated with the LIM domains of zyxin family members is targeting to focal adhesions. Despite this feature of the zyxin family LIM domains, and despite their interaction with p130Cas, it was shown that p130Cas is not required for focal adhesion localization of zyxin and TRIP6 . We also investigated whether LPP was responsible for targeting of Scrib to cell-cell contacts. However, as demonstrated by our experiments, also this appeared not to be the case. In fact, our results indicate that all of the PDZ domains of Scrib are dispensable for targeting the protein to cell-cell contacts. For epithelial cells, these results are in agreement to what has been published in the course of our investigations by Legouis and Jaulin-Bastard et al. , who have shown that a point mutation of a specific proline residue that is located at position 305 in LRR number 13 of human Scrib is enough to abolish membrane localization.
Taken into account that PDZ domains vary in their range and stringency of specificity , it is not excluded that LPP might bind to other PDZ domains than the ones of Scrib. Concerning Scrib, to date, three other proteins have been described that bind to the PDZ domains of Scrib: as mentioned above, the high-risk human papillomavirus E6 protein  interacts with the PDZ domains of human Scrib, whereas the GUKH (guanylate kinase holder) protein was shown to bind to the PDZ domains of Scribble at Drosophila synapses , and very recently, mammalian Scrib was shown to form a tight complex with the βPIX exchange factor at neuronal presynaptic compartments . These findings raise the possibility that different binding partners of the Scrib PDZ domains, including LPP, can compete with each other for binding to Scrib, and as such play a role in processes in which Scrib is involved.
In this regard, it is worth mentioning that the binding of LPP to Scrib appears to be regulated. In our mitochondrial targeting experiments (Fig. 7), we noticed that the full length wild-type LPP-protein was not targeted to Scrib-coated mitochondria in all cells. In fact, in the majority of these cells, full length wild-type LPP was not recruited by Scrib. We hypothesize that the binding of LPP to Scrib is regulated by an intra- or intermolecular interaction of LPP, as a result of which the carboxy-terminal tail is hidden in such a way that it is not available anymore for binding to Scrib. One piece of information that supports this hypothesis is the observation that, in contrast to full length LPP, the carboxy-terminal region containing only the LIM-domains and the tail but lacking the pre-LIM region, was efficiently recruited to Scrib-coated mitochondria in nearly 100% of the cells examined while carboxy-terminally mutated versions were not recruited (our unpublished results). Our observations are similar to what has been reported for the binding of zyxin to the tumor suppressor warts/LATS1. In in vitro binding experiments, it was demonstrated that parts of the zyxin protein containing LIM domains 1 and 2 efficiently bind to warts/LATS1 while the full length protein does not bind . Based on these findings, the authors speculated that the LIM1/2 domains are masked in full-length zyxin, and that intramolecular and/or intermolecular modifications may regulate the interaction between zyxin and warts/LATS1.
Taken together the fact that LPP shuttles between cell adhesion sites and the nucleus [11, 48], and the evidence that we have provided here that Scrib interacts with LPP, establishes that Scrib is connected to the communication pathway between cell adhesion sites and the nucleus of which LPP is an important element, and suggests that LPP is implicated in Scrib-associated cellular events.
The GFP-LPP construct was described before . A construct expressing Xpress-hScrib-mito was made by cloning the coding region of human Scrib with a mutated stop codon in the pcDNA3.1/His vector (Life Technologies) followed by inserting a DNA fragment encoding the membrane anchor of ActA (LILAMLAIGVFSLGAFIKIIQLRKNN; a kind gift of Evelyne Friederich, Centre de Recherche Public-Santé, Luxembourg) behind the mutated stop codon. All amino acid changes in Scrib and LPP were made, using the QuikChange™ Site-Directed Mutagenesis Kit (Stratagene) according to the supplier's protocols. All synthetic mutations, ligation sites and PCR-amplified regions were verified by sequencing. Protein expression was checked by Western blotting.
Construction and sequencing of a full-length human Scrib cDNA
The KIAA0147 partial cDNA clone was kindly provided by Takahiro Nagase (Kazusa DNA Research Institute, Japan). In order to obtain full-length 5'-cDNA sequences encoding human Scrib, RNA-linker mediated 5'-RACE (RLM-RACE) was performed according to published protocols  using RNA isolated from HEK293 cells. The RLM-anchor primer sequence is: 5'-GGGCATAGGCTGACCCTCGCTGAAA-3'. The gene-specific primers are 1) 5'-CACGTCCAGCTCCACCAGCTGCATG-3' and 2) 5'-GAAGTTGGCCACCTCGGGAGGCAAC-3' (nested). This allowed us to construct a composite cDNA of about 5.1 kb which was completely sequenced (Genbank accession no. AF240677).
Yeast two-hybrid system
The Matchmaker Two-Hybrid System 2 was used (Clontech). All experiments were performed in the yeast reporter strain CG-1945. Bait-constructs were made using the vector pGBT9 (Clontech). The prey-constructs pACT2-AF6, pACT2-Erbin, pACT2-PICK1, and pACT2-PSD95 were kindly provided by Jean-Paul Borg (INSERM, Marseille, France), and were described in Audebert and Navarro et al., (AF6, Erbin, PICK1)  and in Saito et al., (PSD-95) . The prey-constructs pACT2-Syntenin and pACT2-CASK were kindly provided by Pascale Zimmermann (University of Leuven & VIB, Belgium). An oligo(dT)- and randomly primed prey-cDNA library constructed with mRNA from 12.5 day embryonic mice using pACT2 as vector  was kindly provided by Kristin Verschueren and Danny Huylebroeck (University of Leuven & VIB, Belgium).
The prey-library was screened as follows: yeast strain CG-1945, containing a HIS3 and a lacZ reporter gene under the control of promoters containing GAL4-binding sites, was transformed with 66 μg of bait-DNA and 33 μg of prey-library-DNA using a LiAc high efficiency transformation protocol . Transformants were grown for 10 days at 30°C on triple selective (lacking Trp, Leu and His) synthetic dropout (SD---) agar plates containing 5 mM 3-AT (Sigma).
Transformed His+ yeast colonies were restreaked on new SD--- agar plates and grown for another 1 to 2 days. Colony-lift filter assays were performed for the qualitative measurement of β-galactosidase activity according to standard protocols.
Cell culture, stable cell lines and transfections
Cell lines used included CV-1 (ATCC CCL-70), HEK293 (ATCC CRL-1573), 293T (HEK 293 cells expressing the SV40 T-antigen), Jurkat (ATCC TIB-152), and MDCK strain II (Dog normal kidney epithelial cells). Jurkat cells were grown in RPMI 1640 (Life Technologies) supplemented with 10% fetal bovine serum. All other cell lines were grown in DMEM/F12 (1:1) (Life Technologies, Inc.) supplemented with 10% fetal bovine serum. Cells were cultured at 37°C in a humidified CO2 incubator.
Transient transfections were performed using FuGene™ 6 Transfection Reagent (Boehringer Mannheim) according to the supplier's instructions. Cells were incubated at 37°C for 18–24 hours before analysis.
Stable MDCK strain II cell lines were made expressing wild-type and carboxy-terminally mutated human GFP-LPP proteins, wild-type full length Scrib-GFP, or Scrib-GFP lacking all four PDZ-domains. Transfection of MDCK cells was performed using Lipofectamine 2000 Reagent (Life Technologies) according to the manufacturer's instructions. Transfected cells were selected in medium containing 250 μg/ml G418 (Life Technologies), and resistant colonies were isolated 10–14 days later. Individual clones were screened for expression of the respective GFP fusion proteins by Western blotting using a rabbit polyclonal anti-GFP antibody (Tebu Bio).
Mammalian two-hybrid system
Bait-constructs were made using pM-vectors , prey-constructs were made in the pSNATCH-vector . 24 hours upon seeding, semi-confluent HEK293 cells on 24-well plates were transiently cotransfected with 100 ng DNA of a bait-construct, 100 ng DNA of a prey-construct, 250 ng DNA of a luciferase reporter construct and 50 ng of CMV-β-galactosidase DNA (internal control for transfection efficiency). The reporter construct contains the gene encoding the firefly luciferase enzyme, which is under the control of a minimal promoter containing five consecutive GAL4-binding sequences (kindly provided by W. Schaffner and D. Escher, Zürich, Switzerland). Cell lysates were prepared 18 to 24 hours after transfection and assayed for luciferase activity as described previously .
In vitro transcription/translation and GST pull-down assays
All in vitro translation reactions were carried out using the TNT T7 Quick Coupled Transcription/Translation System (Promega) following the manufacturer's instructions. For GST pull-down assays, bacterial expression constructs were made using pGEX-5X vectors (Amersham-Pharmacia Biotech) directing the synthesis of glutathione S-transferase (GST) fusion proteins containing wild-type or mutated forms of human LPP. These fusion proteins were purified according to manufacturer's instructions and verified by SDS-PAGE. GST fusion proteins or GST alone, bound to glutathione-agarose beads, were incubated with in vitro synthesized [35S]-methionine-labelled full length human Scrib protein, or a portion of the human Scrib protein encompassing all four PDZ domains (amino acids 616–1490) (wild-type or mutated) in NENT100 buffer (100 mM NaCl, 20 mM Tris-HCl pH = 7.6, 1 mM EDTA, 0.1% NP-40, protease inhibitors). This mixture was tumbled overnight at 4°C. Subsequently the beads were washed 5 times in 500 μl NENT100 buffer, resuspended in 25 μl SDS-PAGE sample buffer and incubated at 95°C for 5 minutes. Proteins were separated by SDS-PAGE and interacting Scrib was detected by autoradiography.
Scrib-specific antiserum and commercial antibodies
The Scrib-specific polyclonal antiserum Scrib-472 was prepared by Eurogentec by immunization of rabbits with a keyhole limpet hemocyanin (KLH) coupled peptide 1612CSSRRPVRPGRRGLGPVPS1630 (19 C-terminal AA of human Scrib). For the detection of endogenous LPP in MDCKII cells, a LPP-specific monoclonal antibody was used (BD Biosciences, Transduction Laboratories). For the detection of GAL4-fusion proteins in immunocytochemistry, a rabbit polyclonal anti-GAL4 DNA-binding domain antibody (Tebu Bio) was used. Vinculin was detected in cells with a monoclonal anti-vinculin antibody (Sigma, clone hVIN-1), Xpress-tagged proteins with a monoclonal anti-Xpress antibody (Life Technologies). Fluorescently-tagged Alexa-antibodies (Molecular Probes) were used as secondary antibodies for immunofluorescence detection.
SDS-PAGE and Western blotting
Eukaryotic cell extracts were prepared by harvesting the cells in PBS (phosphate buffered saline), and subsequent lysis of the cell pellets in SDS-PAGE sample buffer (60 mM TRIS-HCl pH = 6.8, 12% glycerol, 4% SDS, 5% β-mercapto-ethanol). Protein concentrations in cell extracts were determined using BCA Protein Assay Reagent (Pierce) according to the manufacturer's instructions. 30 μg of proteins of each cell extract were loaded on 5% SDS-polyacrylamide gels. After size-separation, proteins were electrophoretically transferred to PROTEAN Nitrocellulose Membranes (Schleicher and Schuell). ECL Western blotting was performed using Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer Life Sciences) according to the supplier's instructions.
GFP-fluorescence and indirect immunocytochemistry
CV-1 or 293T cells seeded on glass coverslips, and MDCKII cells seeded on glass coverslips or on Transwell-Clear polyester membranes (0.4 μm, Costar) were fixed in 4% formaldehyde for 20 minutes at room temperature followed by three washes in PBS containing 0.1 mM CaCl2 and 0.1 mM MgCl2 (PBS++). For GFP-fluorescence, slides were mounted in vectashield mounting medium (Vector Laboratories, Inc.) and analyzed on a Zeiss Axiophot fluorescence microscope equipped with an RT slider SPOT camera (Diagnostic Instruments, Inc.) using SPOT RT Software v3.4, or by confocal microscopy (MRC-1024 Laser Scanning Confocal Imaging System; Bio-Rad). For indirect immunocytochemistry, after fixation, quenching was performed by incubating the cells for 10 minutes at room temperature in PBS++ containing 50 mM NH4Cl. Cells were then permeabilized with 0.4% Triton-X-100 for 5–10 minutes at room temperature. Unspecific binding was blocked with 0.5% Blocking Reagent (Roche) in PBS++ for 30 minutes at room temperature. Subsequently, the slides were incubated with primary antibodies for 1 hour at room temperature. After washing the cells three times in PBS++, bound primary antibodies were detected with fluorescently labelled secondary antibodies (Molecular Probes) for 30 minutes at room temperature. Following three washes in PBS++, slides were mounted and analyzed as described for GFP-fluorescence.
We thank Wim Keysers, Isabelle Orlans and Nancy Weyns for excellent technical assistance, and Jan Brants, Koen Crombez, and Gisèle De Geest for interesting discussions. This work was supported in part by GOA (Geconcerteerde Onderzoeksacties) grant 2002/10, and by grants from the Cancer Research Program of "Fortis Verzekeringen", the Fund for Scientific Research (F.W.O.-Vlaanderen Krediet Aan Navorsers, 1.5.098.03), the Belgian Federation against Cancer (project A5890), and the University VIS program (project 99/010). Marleen Petit is a Postdoctoral Fellow of the Fund for Scientific Research – Flanders (Belgium) (F.W.O. – Vlaanderen).
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