Figure 1

FGF Signaling Either Inhibits Myeloma Cell Proliferation or Has No Effect. A) Myeloma cell lines were treated with FGF1 (100 ng/ml) and heparin (10 ug/ml) or heparin alone (control) for three days and cell number was determined. B9 cells are a mouse plasmacytoma line stably expressing FGF Receptor 3 wild type (wt) or hyperactive mutant K650E [27]. Error bars represent standard deviation of assays performed in triplicate. Results shown are representative of more than four independent determinations for OPM2 cells and three independent determinations for OPM1 cells; p < .01, Student's paired t test. B) OPM1 and OPM2 cells were treated with FGF1 (10 ng/ml) and heparin (10 ug/ml) or heparin only for three days and DNA content was measured using FACS analysis of ethidium bromide stained nuclei.