- Research article
- Open Access
FGF signaling inhibits the proliferation of human myeloma cells and reduces c-myc expression
© Firme and Bush; licensee BioMed Central Ltd. 2003
- Received: 12 May 2003
- Accepted: 04 December 2003
- Published: 04 December 2003
Multiple myeloma is a cancer of antibody producing plasma cells whose etiology is unknown. FGF signaling has been implicated in myeloma pathogenesis but its precise role remains unclear.
Here, we investigate the biochemical and phenotypic consequences of FGF stimulation in several different human myeloma cell lines. We find that FGF signaling inhibits cell cycle progression in two lines and surprisingly, reduces the expression of c-myc while turning on c-fos. In several other lines, FGF signaling does not affect proliferation rate, including cells harboring translocated FGF Receptor 3. When cells are presented with a growth arrest signal, FGF addition induces cell death.
By showing that FGF signaling inhibits mitogenesis and induces apoptosis, we demonstrate novel effects of activating this ubiquitous signaling pathway in multiple myeloma.
- multiple myeloma
Fibroblast growth factor (FGF) signaling regulates the activity of virtually all higher vertebrate cell types. In addition to controlling normal development and physiology, numerous observations support the concept that FGF signaling is abnormal in most forms of human cancer. A review of this voluminous literature is not possible here, but it should be noted that in many cases FGF signaling inhibits rather than promotes growth of the transformed clone. For example, FGF2 (basic FGF) slows the proliferation of breast cancer cells [1, 2], and low levels of FGF2 are associated with accelerated breast cancer progression and worse prognosis . Similarly, mutations in FGF Receptor 3 (FGFR3) are strongly associated with less aggressive forms of bladder cancer, suggesting that FGF signaling may inhibit proliferation in this malignancy [4, 5]. Finally, recent studies show that FGF2 induces apoptosis in Ewing's sarcoma cells .
Alternatively, in some human cancer models, FGF signaling has no effect on tumor progression. In the SV40 T antigen transgenic model of pancreatic cancer, rigorous genetic analysis using both gain and loss of function alleles of FGF Receptor 4 has demonstrated that FGFR4 has no effect on the growth of transformed islet cells, even though it is invariably expressed in this setting . This finding implies that the defect in cell fate determination seen in cancer is accompanied by the misexpression of genes whose action is neutral with respect to oncogenesis.
Multiple Myeloma (MM) is a neoplasia of transformed plasma cells . Genetic abnormalities, including chromosomal translocations, inversions, and deletions are a common feature of MM . Some of the translocations have been characterized and the genes within the rearranged loci pinpointed. One such translocation is t (4;14) which harbors at least two genes in the displaced interval: Fibroblast Growth Factor Receptor 3 (FGFR3) and Multiple Myeloma Set Protein (MMSET) [10, 11]. The presence of FGFR3 in the translocated region has led to the proposal that FGF signaling promotes oncogenesis in myeloma .
The effect of activating FGF signaling on MM cell proliferation is unclear. The translocation involving FGFR3 is observed infrequently, being detectable in approximately fifteen percent of patients [11, 13–16]. In addition, activating mutations in regulatory domains of FGFR3 have been observed in multiple myeloma cell lines and rarely in patient samples [14, 17]. These mutations generally have been shown to augment the signaling potential of the receptor. Interestingly, recent microarray analysis reveals that FGFR3 mRNA is associated with gene expression patterns seen in early, less aggressive sub-groups of multiple myeloma . Indeed, FGFR3 mRNA expression was never observed in more advanced stages of the disease despite translocation of the FGFR3 genomic locus to the IgH gene. This observation indicates that expression of FGFR3 mRNA is disfavored during disease progression. Outcome analysis shows no substantial difference in the progression of patients with translocated FGFR3 .
Notably, multiple FGF receptors are expressed in both myeloma patient samples and cell lines [19, 20], suggesting that genes in this family other than FGFR3 are important. Although multiple myeloma likely originates from an activated, post-switch B lymphocyte, little data exists on the expression of FGF receptors in mature B cells. One exception is the demonstration that FGF receptor stimulation controls the proliferation of activated normal human peripheral blood B cells . Consistent with a role for FGF signaling in mature B cells, we have found that FGF deficient mice produce more antibody to a Type 1 independent antigen and conversely, antibody production is suppressed in FGF over-expressing mice (manuscript in preparation). Thus, it is likely that FGF receptors play a role in mature B cell function. Finally, in addition to multiple receptors, several different FGF ligands are expressed in patient samples and cell lines. For example, FGF2 expression is higher in the serum of patients with multiple myeloma as compared to controls  and this difference is even larger when bone marrow levels are compared . Recent experiments suggest that one source of the FGF2 may be the myeloma cells themselves .
Here we examine the role of FGF signaling in human multiple myeloma cell lines. We find that stimulation of the FGF pathway either inhibits growth or has no effect despite the presence of translocated FGFR3. Growth inhibition is accompanied by reduced expression of c-myc. Surprisingly, FGF treatment of cells in low serum medium stimulates cell death.
To investigate how FGF signaling influences cell cycle progression, we examined the cell cycle profile by FACS analysis of DNA content. We observed that progression through all phases of the cell cycle is retarded by treatment with FGF1, as reflected by a shift to the left in the number of cells with a given fluorescence intensity (Fig. 1b). We found three-fold fewer cells in the G2-M phase of the cell cycle after three days in the presence of FGF1 (region A, fig. 1b). These data demonstrate that FGF signaling slows cell cycle progression in human myeloma cells.
We have investigated the role of FGF signaling in human multiple myeloma cell lines. Examination of numerous lines reveals that FGF treatment either reduces cell number or has no effect. Moreover, when OPM2 cells are presented with a strong growth arrest signal by culturing in low serum medium, FGF addition stimulates apoptosis. These studies demonstrate that the function of FGF signaling in multiple myeloma is more complex than previously appreciated. Since reduced proliferation is observed in two different cell lines derived from the same patient, FGF signaling may inhibit mitogenesis in vivo and thus retard disease progression. This hypothesis is consistent with the silencing of FGFR3 mRNA expression in advanced myeloma, even though the FGFR3 locus is translocated to the actively expressed IgH region . In addition, since FGF fails to inhibit proliferation in most myeloma cell lines despite expression of other FGF receptors [19, 20], FGF-induced growth inhibition may need to be silenced for the transformed clone to expand. Since most myeloma cell lines are derived from cells which have escaped the bone marrow, we speculate that FGF signaling may serve to inhibit proliferation in early stages of the disease, when the transformed clone is confined to the bone marrow microenvironment. Although we can not exclude the possibility that FGF signaling may stimulate proliferation in multiple myeloma cells at some stage of their development, our data indicate that no effect or slower proliferation are more likely outcomes. Studies with primary myeloma cells may shed further light on this issue.
We find that FGF-induced growth inhibition is accompanied by reduced c-MYC expression. To our knowledge, this is the first demonstration of a reduction in c-MYC expression by a classical tyrosine kinase growth factor since FGF stimulation has the opposite effect in many other cell types. Aberrations at the c-myc locus are almost always observed in multiple myeloma cell lines, implying that cis-deregulation is required for growth. In OPM2 cells, the c-myc gene is fused to the IgH locus . Despite this abnormality, it is surprising to observe that FGF stimulation overcomes whatever destabilizing effect it introduces and suppresses c-MYC expression. While activation of the c-myc pathway may not be an initiating event in multiple myeloma, numerous studies have revealed that c-myc contributes to many different human cancers, including multiple myeloma. Therefore, signals which counteract c-MYC expression are of paramount importance.
Studies of the effect of activating the FGFR3 pathway in both fibroblasts and hematopoietic cells have shown that FGFR3 poorly stimulates proliferation. For example, despite abundant expression, both wild type and hyperactive mutant alleles of FGFR3 were unable to transform NIH 3T3 cells . In another study, only the K650E allele produced tumors in nude mice while the wild-type receptor was inert . Recently, a novel FGFR3 mutation (G380D) was shown to weakly transform NIH 3T3 cells. This mutant displayed a similar potency to the K650E allele and was more than 300 times less active than the standard receptor tyrosine kinase/oncogene trk . In BaF3 pro B cells, overexpression of FGFR1 stimulates DNA synthesis while overexpression of FGFR3 does not [24, 26].
On the other hand, the K650E allele has been shown to induce pre-B cell tumors when retrovirally expressed in the bone marrow of transgenic mice . While this is the strongest evidence to date that FGFR3 can positively affect proliferation in hematopoietic cells, over-expression experiments using retroviral vectors which are not easily down regulated might best be interpreted with caution. Indeed, the very early pre-B cell phenotype of the tumors in this report raise the possibility that FGF signaling may regulate immature rather than mature B cell proliferation.
Why does FGF have no effect on the rate of cell division of some myeloma cell lines containing the 4;14 translocation, such as KMS11 and UTMC2? One possibility is that early steps in signaling are not activated normally as a result of mutation. For example, previous studies in plasmacytoma cell lines have demonstrated a block in TGF beta signaling due to inefficient receptor binding at the cell surface . Another possibility is that early steps in FGF signaling are activated but later steps are blocked. To assay for defects in the signal transduction pathway, we examined expression of c-fos in response to FGF stimulation. Since c-fos is normally induced, we conclude that the FGF pathway leading to changes in gene expression remains unperturbed by mutation.
The induction of apoptosis by FGF stimulation in OPM2 cells reveals that in sharp contrast to acting as a survival factor, FGF signaling transmits a death signal. Death induction by classical growth and survival factors is not without precedent. FGF signaling in human chondrocytes expressing the K650E allele activates cell death without affecting proliferation rate . Moreover, in FGF2 transgenic mice, FGF induced cell death is inhibited by reducing stat1 gene dosage, suggesting that stat1 mediated growth inhibition may precede the execution of the apoptotic program . Notably, FGF stimulation of multiple myeloma cell lines harboring t(4;14) does not stimulate stat activity . The accumulating evidence for death induction by receptor tyrosine kinases implies that further investigation of this phenomenon may reveal new pathways of cell suicide. Future research will be directed at understanding in greater detail the molecular mechanism by which FGF signaling controls these processes.
We have demonstrated that FGF signaling inhibits the proliferation of human myeloma cells.
Inhibition is accompanied by reduced DNA synthesis and surprisingly, reduced expression of the proto-oncogene c-myc. In many other cell lines FGF signaling did not affect proliferation rate, including cell lines with translocated FGFR3. Under growth suppressed conditions, FGF signaling induced apoptosis. Taken together, our data reveal unanticipated effects of FGF signaling in multiple myeloma and suggest novel conclusions concerning its role in the oncogenic process.
OPM1 cells were from Edward Thompson (University of Texas). UTMC2, OPM2, KMS11, and JIM3 cells were the kind gift of P. Leif Bergsagel (Cornell University Medical College). KMS18 cells were from Antonino Neri (University of Milan), ARP-1 cells were from Joshua Epstein (University of Arkansas), and ANBL6 lines were from Diane Jelinek (Mayo Clinic). B9 plasmacytoma cells were the generous gift of A. Keith Stewart (University of Toronto) and were supplemented with 1–10 ng/ml Il-6. All other lines were from ATCC. All cell lines were cultured in RPMI 1640 media with 10% FCS, except UTMC2 cells which were supplemented with 1–10 ng/ml Il-6 (R and D Systems). For serum starvation experiments, cells were replated in media lacking serum but containing insulin, transferrin and selenium (Gibco Serum Supplement) for twenty four hours. Recombinant human FGF1 and 2 were from R and D (Minnesota).
Cell Proliferation Experiments
Cell number following FGF stimulation during log phase growth was determined either by counting the number of trypan blue negative cells or by Coulter Counter with identical results. For B9 plasmacytoma derivatives, proliferation rate was measured in the presence of 1–10 ng/ml Il-6. For thymidine incorporation studies, cells were incubated in serum free medium for 24 hours in the presence or absence of FGF1. Cells were harvested 24 hours later. 4–16 hours before harvest, 0.5 uCi H3 thymidine per 100 ul was added. Experiments were performed in triplicate and counted on a Beta plate reader (LKB Wallac).
Cell Cycle Experiments
FACS analysis was performed on nuclei isolated by NP-40 lysis . Briefly, cells were centrifuged at 1000 × G and incubated in NP-40 lysis buffer with RNAse (10 ug/ml) at room temperature for 30 minutes followed by citric acid/sucrose buffer containing ethidium bromide. Successful isolation of nuclei was confirmed by microscopic examination. Cell cycle analysis was performed on a Coulter Model XL flow cytometer, gating on signals indicative of intact, non-clumped nuclei.
Immunoblotting and Northern analysis
Cells in log phase growth were harvested by centrifugation and lysed at 4° in RIPA buffer (20 mM Tris pH 8.0, 137 mM NaCl, 10% v/v glycerol, 1% Nonidet P-40, 0.1% Sodium Dodecyl Sulfate, 0.5% Sodium Deoxycholate, 2 mM EDTA) containing protease inhibitors (aprotinin, leupeptin, pepstatin and PMSF). Lysates were clarified by centrifugation at 14, 000 × G. Protein concentration was determined using the BCA method (Pierce). c-MYC was visualized by ECL (Amersham) using the monoclonal C-33 antibody at 1:1000 (Santa Cruz) and HRP coupled Goat anti-Mouse secondary at 1:10,000 (Jackson).
For studies of fos and myc mRNA expression, total RNA was isolated by guanidium isothiocyanate lysis (Trizol, GIBCO/BRL) or Qiagen mRNA easy (Qiagen) and separated on 1% agarose gels containing formaldehyde. RNA was transferred to nylon membranes (Schleicer and Schuell) and probed with random primer generated P32 labeled probes from gel purified fragments of mouse cDNA encoding c-fos and c-myc. Washing was performed at a minimum stringency of 55°C in 0.1X SSC and 0.1% SDS.
Supported by the T.J. Martell Fund for Research in Myeloma and The Leukemia Society of America. We are grateful to the late Joseph Michaeli for support in the early stages of this work and to Dr. Aaron Marcus in the later stages. Special thanks to Roger Pearse for insightful critical input and Sheik Baksh for expert technical assistance.
- Korah RM, Sysounthone V, Golowa Y, Wieder R: Basic fibroblast growth factor confers a less malignant phenotype in MDA-MB-231 human breast cancer cells. Cancer Res. 2000, 60: 733-740.PubMedGoogle Scholar
- Korah RM, Sysounthone V, Scheff E, Wieder R: Intracellular FGF-2 promotes differentiation in T-47D breast cancer cells. Biochem Biophys Res Commun. 2000, 277: 255-260. 10.1006/bbrc.2000.3655.View ArticlePubMedGoogle Scholar
- Yiangou C, Gomm JJ, Coope RC, Law M, Luqmani YA, Shousha S, Coombes RC, Johnston CL: Fibroblast growth factor 2 in breast cancer: occurrence and prognostic significance. Br J Cancer. 1997, 75: 28-33.PubMed CentralView ArticlePubMedGoogle Scholar
- van Rhijn BW, Lurkin I, Radvanyi F, Kirkels WJ, van der Kwast TH, Zwarthoff EC: The fibroblast growth factor receptor 3 (FGFR3) mutation is a strong indicator of superficial bladder cancer with low recurrence rate. Cancer Res. 2001, 61: 1265-1268.PubMedGoogle Scholar
- Billerey C, Chopin D, Aubriot-Lorton MH, Ricol D, Gil Diez de Medina S, Van Rhijn B, Bralet MP, Lefrere-Belda MA, Lahaye JB, Abbou CC, Bonaventure J, Zafrani ES, van der Kwast T, Thiery JP, Radvanyi F: Frequent FGFR3 mutations in papillary non-invasive bladder (pTa) tumors. Am J Pathol. 2001, 158: 1955-1959.PubMed CentralView ArticlePubMedGoogle Scholar
- Westwood G, Dibling BC, Cuthbert-Heavens D, Burchill SA: Basic fibroblast growth factor (bFGF)-induced cell death is mediated through a caspase-dependent and p53-independent cell death receptor pathway. Oncogene. 2002, 21: 809-824. 10.1038/sj.onc.1205128.View ArticlePubMedGoogle Scholar
- Olson DC, Deng C, Hanahan D: Fibroblast growth factor receptor 4, implicated in progression of islet cell carcinogenesis by its expression profile, does not contribute functionally. Cell Growth Differ. 1998, 9: 557-564.PubMedGoogle Scholar
- Kuehl WM, Bergsagel PL: Multiple myeloma: evolving genetic events and host interactions. Nat Rev Cancer. 2002, 2: 175-187. 10.1038/nrc746.View ArticlePubMedGoogle Scholar
- Bergsagel PL, Kuehl WM: Chromosome translocations in multiple myeloma. Oncogene. 2001, 20: 5611-5622. 10.1038/sj.onc.1204641.View ArticlePubMedGoogle Scholar
- Richelda R, Ronchetti D, Baldini L, Cro L, Viggiano L, Marzella R, Rocchi M, Otsuki T, Lombardi L, Maiolo AT, Neri A: A novel chromosomal translocation t(4; 14)(p16.3; q32) in multiple myeloma involves the fibroblast growth-factor receptor 3 gene. Blood. 1997, 90: 4062-4070.PubMedGoogle Scholar
- Chesi M, Nardini E, Brents LA, Schrock E, Ried T, Kuehl WM, Bergsagel PL: Frequent translocation t(4;14)(p16.3;q32.3) in multiple myeloma is associated with increased expression and activating mutations of fibroblast growth factor receptor 3. Nat Genet. 1997, 16: 260-264.PubMed CentralView ArticlePubMedGoogle Scholar
- Chesi M, Bergsagel PL, Kuehl WM: The enigma of ectopic expression of FGFR3 in multiple myeloma: a critical initiating event or just a target for mutational activation during tumor progression. Curr Opin Hematol. 2002, 9: 288-293. 10.1097/00062752-200207000-00005.View ArticlePubMedGoogle Scholar
- Malgeri U, Baldini L, Perfetti V, Fabris S, Vignarelli MC, Colombo G, Lotti V, Compasso S, Bogni S, Lombardi L, Maiolo AT, Neri A: Detection of t(4;14)(p16.3;q32) chromosomal translocation in multiple myeloma by reverse transcription-polymerase chain reaction analysis of IGH-MMSET fusion transcripts. Cancer Res. 2000, 60: 4058-4061.PubMedGoogle Scholar
- Intini D, Baldini L, Fabris S, Lombardi L, Ciceri G, Maiolo AT, Neri A: Analysis of FGFR3 gene mutations in multiple myeloma patients with t(4;14). Br J Haematol. 2001, 114: 362-364. 10.1046/j.1365-2141.2001.02957.x.View ArticlePubMedGoogle Scholar
- Rasmussen T, Hudlebusch HR, Knudsen LM, Johnsen HE: FGFR3 dysregulation in multiple myeloma: frequency and prognostic relevance. Br J Haematol. 2002, 117: 626-628. 10.1046/j.1365-2141.2002.03429.x.View ArticlePubMedGoogle Scholar
- Ronchetti D, Greco A, Compasso S, Colombo G, Dell'Era P, Otsuki T, Lombardi L, Neri A: Deregulated FGFR3 mutants in multiple myeloma cell lines with t(4;14): comparative analysis of Y373C, K650E and the novel G384D mutations. Oncogene. 2001, 20: 3553-3562. 10.1038/sj.onc.1204465.View ArticlePubMedGoogle Scholar
- Chesi M, Brents LA, Ely SA, Bais C, Robbiani DF, Mesri EA, Kuehl WM, Bergsagel PL: Activated fibroblast growth factor receptor 3 is an oncogene that contributes to tumor progression in multiple myeloma. Blood. 2001, 97: 729-736. 10.1182/blood.V97.3.729.View ArticlePubMedGoogle Scholar
- Zhan F, Hardin J, Kordsmeier B, Bumm K, Zheng M, Tian E, Sanderson R, Yang Y, Wilson C, Zangari M, Anaissie E, Morris C, Muwalla F, van Rhee F, Fassas A, Crowley J, Tricot G, Barlogie B, Shaughnessy J., Jr.: Global gene expression profiling of multiple myeloma, monoclonal gammopathy of undetermined significance, and normal bone marrow plasma cells. Blood. 2002, 99: 1745-1757. 10.1182/blood.V99.5.1745.View ArticlePubMedGoogle Scholar
- Otsuki T, Yamada O, Yata K, Sakaguchi H, Kurebayashi J, Nakazawa N, Taniwaki M, Yawata Y, Ueki A: Expression of fibroblast growth factor and FGF-receptor family genes in human myeloma cells, including lines possessing t(4;14)(q16.3;q32. 3) and FGFR3 translocation. Int J Oncol. 1999, 15: 1205-1212.PubMedGoogle Scholar
- Bisping G, Leo R, Wenning D, Dankbar B, Padro T, Kropff M, Scheffold C, Kroger M, Mesters RM, Berdel WE, Kienast J: Paracrine interactions of basic fibroblast growth factor and interleukin-6 in multiple myeloma. Blood. 2003, 101: 2775-2783. 10.1182/blood-2002-09-2907.View ArticlePubMedGoogle Scholar
- Genot E, Petit-Koskas E, Sensenbrenner M, Labourdette G, Kolb JP: Potentiation of the proliferative response of human B lymphocytes to low molecular weight B cell growth factor (LMW-BCGF) by fibroblast growth factors (FGFs). Cell Immunol. 1989, 122: 424-439.View ArticlePubMedGoogle Scholar
- Sezer O, Jakob C, Eucker J, Niemoller K, Gatz F, Wernecke K, Possinger K: Serum levels of the angiogenic cytokines basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in multiple myeloma. Eur J Haematol. 2001, 66: 83-88. 10.1034/j.1600-0609.2001.00348.x.View ArticlePubMedGoogle Scholar
- Di Raimondo F, Azzaro MP, Palumbo G, Bagnato S, Giustolisi G, Floridia P, Sortino G, Giustolisi R: Angiogenic factors in multiple myeloma: higher levels in bone marrow than in peripheral blood. Haematologica. 2000, 85: 800-805.PubMedGoogle Scholar
- Ornitz DM, Xu J, Colvin JS, McEwen DG, MacArthur CA, Coulier F, Gao G, Goldfarb M: Receptor specificity of the fibroblast growth factor family. J Biol Chem. 1996, 271: 15292-15297. 10.1074/jbc.271.25.15292.View ArticlePubMedGoogle Scholar
- Katagiri S, Yonezawa T, Kuyama J, Kanayama Y, Nishida K, Abe T, Tamaki T, Ohnishi M, Tarui S: Two distinct human myeloma cell lines originating from one patient with myeloma. Int J Cancer. 1985, 36: 241-246.View ArticlePubMedGoogle Scholar
- Naski MC, Wang Q, Xu J, Ornitz DM: Graded activation of fibroblast growth factor receptor 3 by mutations causing achondroplasia and thanatophoric dysplasia. Nat Genet. 1996, 13: 233-237.View ArticlePubMedGoogle Scholar
- Plowright EE, Li Z, Bergsagel PL, Chesi M, Barber DL, Branch DR, Hawley RG, Stewart AK: Ectopic expression of fibroblast growth factor receptor 3 promotes myeloma cell proliferation and prevents apoptosis. Blood. 2000, 95: 992-998.PubMedGoogle Scholar
- Eisenman RN: Deconstructing myc. Genes Dev. 2001, 15: 2023-2030. 10.1101/gad928101.View ArticlePubMedGoogle Scholar
- Shou Y, Martelli ML, Gabrea A, Qi Y, Brents LA, Roschke A, Dewald G, Kirsch IR, Bergsagel PL, Kuehl WM: Diverse karyotypic abnormalities of the c-myc locus associated with c- myc dysregulation and tumor progression in multiple myeloma. Proc Natl Acad Sci U S A. 2000, 97: 228-233. 10.1073/pnas.97.1.228.PubMed CentralView ArticlePubMedGoogle Scholar
- Avet-Loiseau H, Gerson F, Magrangeas F, Minvielle S, Harousseau JL, Bataille R: Rearrangements of the c-myc oncogene are present in 15% of primary human multiple myeloma tumors. Blood. 2001, 98: 3082-3086. 10.1182/blood.V98.10.3082.View ArticlePubMedGoogle Scholar
- Hardin J, MacLeod S, Grigorieva I, Chang R, Barlogie B, Xiao H, Epstein J: Interleukin-6 prevents dexamethasone-induced myeloma cell death. Blood. 1994, 84: 3063-3070.PubMedGoogle Scholar
- Lichtenstein A, Tu Y, Fady C, Vescio R, Berenson J: Interleukin-6 inhibits apoptosis of malignant plasma cells. Cell Immunol. 1995, 162: 248-255. 10.1006/cimm.1995.1076.View ArticlePubMedGoogle Scholar
- Webster MK, Donoghue DJ: Enhanced signaling and morphological transformation by a membrane- localized derivative of the fibroblast growth factor receptor 3 kinase domain. Mol Cell Biol. 1997, 17: 5739-5747.PubMed CentralView ArticlePubMedGoogle Scholar
- Li Z, Zhu YX, Plowright EE, Bergsagel PL, Chesi M, Patterson B, Hawley TS, Hawley RG, Stewart AK: The myeloma-associated oncogene fibroblast growth factor receptor 3 is transforming in hematopoietic cells. Blood. 2001, 97: 2413-2419. 10.1182/blood.V97.8.2413.View ArticlePubMedGoogle Scholar
- Amoroso SR, Huang N, Roberts AB, Potter M, Letterio JJ: Consistent loss of functional transforming growth factor beta receptor expression in murine plasmacytomas. Proc Natl Acad Sci U S A. 1998, 95: 189-194. 10.1073/pnas.95.1.189.PubMed CentralView ArticlePubMedGoogle Scholar
- Legeai-Mallet L, Benoist-Lasselin C, Delezoide AL, Munnich A, Bonaventure J: Fibroblast growth factor receptor 3 mutations promote apoptosis but do not alter chondrocyte proliferation in thanatophoric dysplasia. J Biol Chem. 1998, 273: 13007-13014. 10.1074/jbc.273.21.13007.View ArticlePubMedGoogle Scholar
- Sahni M, Raz R, Coffin JD, Levy D, Basilico C: STAT1 mediates the increased apoptosis and reduced chondrocyte proliferation in mice overexpressing FGF2. Development. 2001, 128: 2119-2129.PubMedGoogle Scholar
- Giaretti W, Nusse M: Light scatter of isolated cell nuclei as a parameter discriminating the cell-cycle subcompartments. Methods Cell Biol. 1994, 41: 389-400.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.