Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6
© Anthonio et al; licensee BioMed Central Ltd. 2009
Received: 20 February 2009
Accepted: 17 August 2009
Published: 17 August 2009
Peroxisomes execute diverse and vital functions in virtually every eukaryote. New peroxisomes form by budding from pre-existing organelles or de novo by vesiculation of the ER. It has been suggested that ADP-ribosylation factors and COPI coatomer complexes are involved in these processes.
Here we show that all viable Saccharomyces cerevisiae strains deficient in one of the small GTPases which have an important role in the regulation of vesicular transport contain functional peroxisomes, and that the number of these organelles in oleate-grown cells is significantly upregulated in the arf1 and arf3 null strains compared to the wild-type strain. In addition, we provide evidence that a portion of endogenous Arf6, the mammalian orthologue of yeast Arf3, is associated with the cytoplasmic face of rat liver peroxisomes. Despite this, ablation of Arf6 did neither influence the regulation of peroxisome abundance nor affect the localization of peroxisomal proteins in cultured fetal hepatocytes. However, co-overexpression of wild-type, GTP hydrolysis-defective or (dominant-negative) GTP binding-defective forms of Arf1 and Arf6 caused mislocalization of newly-synthesized peroxisomal proteins and resulted in an alteration of peroxisome morphology.
These observations suggest that Arf6 is a key player in mammalian peroxisome biogenesis. In addition, they also lend strong support to and extend the concept that specific Arf isoform pairs may act in tandem to regulate exclusive trafficking pathways.
Peroxisomes are a diverse group of organelles which accommodate many activities related to lipid metabolism. In mammals, these organelles harbor a number of essential metabolic functions including ether phospholipid biosynthesis, fatty acid α – and β-oxidation, and glyoxylate detoxification . The size, number and enzyme content of peroxisomes can be affected by various environmental, metabolic, and developmental factors [2–4]. Peroxisomes are thought to arise de novo from the endoplasmic reticulum (ER) [5–8] or multiply by fission of pre-existing peroxisomes [3, 9, 10]. The relative contribution of both pathways to the total number of peroxisomes in wild-type cells is not yet clear [7, 10, 11], and the molecular mechanisms governing these processes are only beginning to be unraveled .
Studies in different model systems have demonstrated that the Pex11p family of peroxisomal membrane proteins (PMPs), a select set of dynamin-related proteins (DRPs), and Fis1 and Mff – two putative adaptors for DRPs – coordinately control the shape, size and number of peroxisomes in a cell [3, 11, 13–15]. The Pex11 proteins are supposed to be involved in the elongation and tubulation of pre-existing peroxisomes, whereas the DRPs, Fis1, and Mff are thought to catalyze the final fission event [3, 11, 15]. It has been put forward also that ADP-ribosylation factors and coatomer proteins are involved in the biosynthetic process of the peroxisomal membrane . Arguments in favor of this hypothesis are the observations that (i) Arf1, Arf6, and COPI coatomer proteins can bind to highly purified rat liver peroxisomes [17–19], (ii) expression of a temperature-sensitive ε-COP subunit in CHO cells at non-permissive temperature exhibited a dramatic change in peroxisome morphology , and (iii) the S. cerevisiae orthologues of mammalian Arf1 (ScArf1) and Arf6 (ScArf3) regulate peroxisome division up and down, respectively . Also, peroxisomal Arf/COPI might be involved in the retrieval of cargo from peroxisomes back to the ER [12, 16, 20]. The observation that a dominant-negative mutant of Arf1 inhibits the transport pathway of tomato bushy stunt virus 33 kDa replication protein from peroxisomes to the ER in Nicotiana tabacum cells supports this hypothesis .
Arf1 and Arf6 are – together with Sar1 – the most comprehensively studied proteins of the ADP-ribosylation factor (ARF) family . Members of this group of small GTPases are believed to control vesicular trafficking and organelle structure by recruiting coat proteins, to function as regulators of phospholipid metabolism, and to modulate cytoskeletal organization [23, 24]. Arf1 has been shown to function in the GTP-dependent recruitment of COPI coatomer to budding transport vesicles that move from the Golgi to the ER . In addition, it has been shown that ARF1-GTP regulates the recruitment of clathrin to late Golgi and endosome compartments through binding with heterotetrameric adaptor protein complexes as well as monomeric Golgi-localized γ-ear-containing ARF-binding proteins . Arf6, a protein predominantly associated with the plasma membrane, is thought to regulate endosomal membrane delivery and traffic, membrane raft trafficking, membrane recycling during phagocytosis, and actin remodeling at the cell periphery [23, 24]. In this study, we dissect the potential role of small GTPases, and especially of Arf6, in peroxisome biogenesis.
Yeast cells deficient in Arf1 or Arf3 exhibit a pronounced increase in peroxisome number on oleate compared to wild-type cells
Copurification of Arf proteins with rat liver peroxisomes
Arf6 is not essential for peroxisome biogenesis in mouse hepatocytes
Co-overexpression of Arf1 and Arf6 variants impairs peroxisomal protein import in mammalian cells
In many instances, the formation of membrane-delimited compartments from pre-existing organelles is regulated by small GTPases of the Rab and Arf family [22, 23, 39, 40]. A growing amount of evidence suggests that monomeric GTP-binding proteins may also play a role in peroxisome biogenesis. For example, mass spectrometry-based quantitative proteomic profiling studies have shown that in yeast Rho1 is recruited to peroxisomes upon their induction by oleate, and it has been suggested that this protein regulates the assembly state of actin on the peroxisome membrane, thereby controlling peroxisome membrane dynamics and biogenesis . Recently, it has also been shown that MYA2, an isoform of Arabidopsis thaliana myosin XI, is targeted to peroxisomes through an interaction with AtRab2CA . Furthermore, mammalian Arf1 and Arf6 have been shown to bind to isolated rat liver peroxisomes, and ScArf1 and ScArf3, the yeast orthologues of these proteins, have been implicated in the control of peroxisome proliferation . A similar function has been suggested for Trypanosoma brucei Arf1, a protein which shares characteristics with both Arf1 and Arf6 and has a vital role in the maintenance of the endocytic pathway . Interestingly, no Arf proteins have been identified in proteome studies of yeast and mammalian peroxisomes [41, 44–50]. A possible explanation might be that the relative abundance of these proteins in the analyzed samples is extremely low.
In this study, we initially investigated whether or not yeast strains deficient in Arf1, Arf2, Arf3, Arl1, Cin4, Vps21, Ypt6, Ypt7, Ypt10, Ypt11, Ypt31, Ypt32, Ypt52 or Ypt53 were capable of growing on oleate as a sole carbon source and/or displayed a peroxisomal protein sorting defect. The results of these experiments demonstrated that all these strains, which embody every viable yeast deletion mutant of the Rab and Sar1/Arf subfamilies of small GTPases, contain functional peroxisomes. Interestingly, the average number of peroxisomes in oleate-grown cells was significantly upregulated in the Δarf1 and Δarf3 strains compared to the wild-type strain, and this phenotype was even more pronounced in the double Δarf1Δarf3 deletion mutant. This observation indicates that ScArf1 and ScArf3 dampen peroxisome proliferation, either directly or indirectly. Although this result confirms previous observations that ScArf1 and ScArf3 are implicated in the control of peroxisome proliferation , it is not consistent with the authors' conclusion that ScArf1 is required for the oleate-induced peroxisome proliferation in S. cerevisiae. The reasons for the discrepancy between our results and the results previously presented might be several. For example, while we were making use of an Arf1 deletion mutant, Lay et al.  utilized an Arf1 temperature-sensitive mutant in an Δarf2 background. Also, while these authors analyzed the peroxisome number per cell after 6 hours of growth on oleate, we analyzed the cells after 5 or 9 days of growth on oleate. Note that after transfer of the cells to oleate-containing medium, the number of peroxisomes per cell continuously increased as a function of time for at least 14 days, in the Δarf1 and Δarf3 strains compared to the wild-type strain (data not shown). At this point, it is not clear how Arf1 and Arf3 suppress oleate-induced peroxisome proliferation in S. cerevisiae. One possibility might be that these proteins are involved in the generation of peroxisome-derived vesicles, which transport ER-derived factors from peroxisomes back to the ER [7, 8, 12, 16] or deliver metabolites, lipids, or (mistargeted) proteins to mitochondria . Disturbances in such pathways would result in an increase in peroxisome number.
We next investigated whether or not endogenous Arf proteins were associated with the membrane of rat liver peroxisomes. In a first series of experiments, we obtained evidence that 1D9, a mouse monoclonal antibody with specificity for mammalian Arf1, Arf2, Arf3, Arf5, Arf6, and – to a lesser extent – Arf4 , recognized a 21 kDa band in highly purified peroxisomal membrane preparations. We could show that this 21 kDa protein was associated with the outside face of the peroxisomal membrane (as determined by immuno-electron microscopy), and identify this protein as Arf6 (as determined with Arf-specific antibodies). This protein, which is considered to be the mammalian orthologue of yeast Arf3, has been reported to act in a wide range of processes, including endocytosis, cytokinesis, phagocytosis, and the organization of the actin cytoskeleton . Our observation that endogenous Arf6 is also associated with peroxisomes may seem surprising given that the subcellular localization of this protein has already been documented in several reports [30, 52–54]. However, as (hepatic) peroxisomes occupy less than 2% of the cell volume , a partial (and transient) association of Arf6 with these organelles may have been easily missed. Also, as the strength of the signal and its detection depend heavily on the amount of protein and may be easily masked by stronger signals, the methods employed might not be sensitive enough. No evidence could be obtained for the association of any other endogenous Arf protein with the peroxisomal membrane. However, these results should be considered with caution as such proteins may dissociate during the purification procedure and be present on the peroxisomal membrane in concentrations below the detection limits of the employed Arf-specific antibodies.
To study the function of Arf6 in peroxisome biogenesis, we first examined the number of peroxisomes, peroxisomal morphology, and the location of (newly-synthesized) peroxisomal membrane and matrix proteins in (i) Ptk2 cells transiently overexpressing either wild-type Arf6 or a mutant defective in GTP-binding (Arf6T27N) or GTP-hydrolysis (Arf6Q67L) and (ii) Arf6-/- fetal mouse hepatocytes . Neither the overexpression of Arf6 variants nor the absence of Arf6 resulted in a detectable peroxisomal phenotype. Together, these observations suggest that peroxisome-bound Arf6 alone is not essential for peroxisome biogenesis in mammalian cells. The lack of a phenotypic defect is most probably the result of functional redundancy among individual Arf proteins , and since it has been reported that exogenously added Arf1 can bind to isolated rat liver peroxisomes , we decided to carry out another series of experiments in which Arf6WT, Arf6T27N, or Arf6Q67L were transiently co-overexpressed with either wild-type Arf1 or a mutant defective in GDP-binding (Arf1T31N,) or GTP-hydrolysis (Arf1Q71L). These experiments showed that a combined overexpression of Arf1 and Arf6 variants resulted in a (partial) mislocalization of newly-synthesized peroxisomal reporter proteins and an altered peroxisome morphology. Importantly, the intracellular transport pathway of HsLK2-Myc-His, a human mitochondrial multisubstrate lipid kinase , was not disturbed. Interestingly, combinations with Arf1T31N and Arf6T27N consistently displayed the strongest phenotypes. Similar observations were observed in CHO and HepG2 cells (data not shown). These mutants are thought to render endogenous Arf1 and Arf6 inactive, presumably by sequestering a select set of guanine nucleotide exchange factors [35, 52]. Since a similar, but less drastic, phenotype was observed when other Arf6 and Arf1 variants were expressed, and Arf3, Arf4, and Arf5 could not substitute for Arf1, it is unlikely that the (partial) mislocalization of the peroxisomal reporter proteins is the result of indirect changes in the level of activation of other Arf subtypes. In addition, our observation that a similar phenotype was obtained with all Arf1 and Arf6 variants also suggests the necessity for a completion of the GDP/GTP cycle of these proteins to achieve their potential function in peroxisome biogenesis [, and references therein]. In summary, these findings – which suggest that Arf1 and Arf6 act in tandem to regulate a peroxisomal trafficking pathway – lend strong support to and extend the hypothesis that the cooperation of two Arf proteins at the same subcellular location is a general feature of Arf signaling .
To date, only one group has studied the potential relationship between ADP-ribosylation factors and peroxisome formation in mammalian cells, and shown that exogenously added Arf1 and Arf6 can bind to isolated rat liver peroxisomes [17, 19]. In this work, we have extended these findings by showing that (i) also endogenous Arf6 associates with the cytoplasmic side of the peroxisomal membrane, and (ii) a combined overexpression of Arf6 and Arf1 variants selectively impairs peroxisomal protein import and alters peroxisome morphology. The molecular mechanisms underlying these phenotypic alterations remain to be clarified, since these Arf proteins do not interact directly with any of the currently identified human peroxins as tested with yeast- and bacterial two-hybrid assays (data not shown). Nevertheless, based on (i) a recent report showing that active Arf6 can recruit ARNO – a soluble Arf1 GTPase-activating guanine nucleotide exchange factor – to the plasma membrane , (ii) a growing body of evidence supporting the existence of vesicular trafficking pathways between peroxisomes and other subcellular compartments [8, 51], and (iii) the observation that Arf1 is involved in the transport pathway of tomato bushy stunt virus 33 kDa replication protein from peroxisomes to the ER in Nicotiana tabacum cells , it is tempting to speculate that activated peroxisomal Arf6 is involved in the formation of peroxisome-derived vesicles by serving as an adaptor for the recruitment of not yet identified guanine nucleotide exchange factor(s) to sites at the peroxisomal membrane which in turn may lead to Arf1 activation and cytosolic coat protein recruitment. Depending on the functional redundancy among the Arf proteins and the role of the peroxisome-derived vesicles in different organisms, the inability of a cell to form such vesicles may result in an increase in peroxisome number or a block in the formation of new organelles.
In this study, we provide evidence that endogenous Arf6, the mammalian orthologue of S. cerevisiae Arf3, can tightly associate with the outside face of the peroxisomal membrane. We also demonstrated that a combined overexpression of Arf6 and Arf1 variants caused mislocalization of newly-synthesized peroxisomal proteins and resulted in an alteration of peroxisome morphology. These observations suggest that Arf6 – albeit not essential – is a key player in mammalian peroxisome biogenesis. In addition, these findings extend the concept that specific Arf isoform pairs may act in tandem to regulate exclusive trafficking pathways. The precise mechanism by which Arf6 functions in peroxisome biogenesis remains to be determined.
Male Wistar rats (weighing 200–250 g), Swiss-Webster mice, and New Zealand White rabbits were kept in a constant light-dark cycle on a standard laboratory diet. The rats were fasted 16 hours before sacrifice. Arf6+/- C57BL/6 mice were maintained in a pathogen-free facility as described elsewhere . To study the effect of a peroxisome proliferator on the numerical abundance, structure, and protein import competence of peroxisomes in Arf6-/- fetal hepatocytes, pregnant Arf6+/- mice were fed a diet containing 0.3% (w/w) clofibrate beginning at day 6.5 of gestation . All animal experiments were approved by the local institutional ethics committees.
Cloning vectors were obtained from Qiagen (pQE30), Clontech (pEGFP-N1, pIRES2-EGFP) or Stratagene (pCMV-Tag4A, B), and the oligonucleotides synthesized for this study were from Eurogentec. PCR applications were performed routinely using Pfx DNA polymerase (Invitrogen). Restriction enzymes were purchased from TaKaRa. The Escherichia coli strain Top10F' (Invitrogen) was used for all DNA manipulations. Full-length cDNAs coding for human (Homo sapiens, Hs) Arf1 (accession number NM_001649), Arf3 (accession number NM_001659), Arf4 (accession number NM_001660), Arf5 (accession number NM_001662), and Arf6 (accession number NM_001663) were obtained from the I.M.A.G.E. consortium (clones 3463523, 2967578, 6157009, 6720876, and 5212700, respectively) . The mammalian expression vectors coding for Bos Taurus (Bt) Arf1WT-HA, HsArf1T31N-HA, or HsArf1Q71L-HA  were obtained from Addgene (plasmids 10830, 10833, and 10832, respectively). The plasmids coding for HsArf6-HA, HsArf6T27N-HA, or HsArf6Q67L-HA were kindly provided by Dr. P. Chavrier (Institut Curie, Paris, France). The yeast expression vector encoding EGFP-PTS1 (pJR233) was kindly provided by Dr. A. Hartig (University of Vienna, Austria). The mammalian expression plasmids pTW360 (coding for HsPMP34-Myc-His) and pSG005 (coding for HsLK2-Myc-His) have been described elsewhere [56, 62]. The bicistronic expression plasmids coding for EGFP-PTS1 and non-tagged HsArf1T31N (pEA42), HsArf6WT (pEA38) or HsArf6Q67L (pEA91) were constructed by amplifying the corresponding cDNAs by PCR (Arf1 primers: 5'-gtttgagatctcatggggaacatcttcgcc-3' (HsArf1F1) and 5'-caaaagtcgactcacttctggttccggagc-3' (HsArfR4); Arf6 primers: 5'-gtttgagatctgatggggaaggtgctatcc-3' (HsArf6f1) and 5'-gtgtggtcgactcattaagatttgtagttagagg-3' (HsArf6r4)), and cloning the Bgl II/Sal I-digested PCR products into the multiple cloning site of pIRES2-EGFP-PTS1 (pMF891)  digested with the same restriction enzymes. The bicistronic plasmid coding for EGFP-PTS1 and non-tagged HsArf6T27N (pEA43) was constructed by fusion PCR. In a first PCR reaction, two PCR fragments (template: pEA38; primers: 5'-ggactttcctacttggcag-3' (pIRES2-EGFP-f1) and 5'-ggattgtattcttgccggccgcgtcc-3' (HsArf6T27Nr1) (fragment 1), 5'-ccggcaagaatacaatcctgtacaagttgaag-3' (HsArf6T27Nf1) and HsArf6r4 (fragment 2)) were generated. These PCR fragments were fused and used as a template in a second PCR reaction (primers: HsArf6f1, HsArf6r4). After digestion with Bgl II and Sal I, the fusion fragment was subcloned into the Bgl II/Sal I-digested pIRES2-EGFP-PTS1 vector. The mammalian expression vectors coding for HsArf3-FLAG (pMF1599), HsArf4-FLAG (pMF1598) or Arf5-FLAG (pMF1577) were constructed by transferring the Bam HI/Bgl II-digested (pMF1577, pMF1599) or Bgl II/Sal I-digested (pMF1598) cDNA inserts from pEA51, pEA35, and pEA52 into the Bam HI-digested pCMV-Tag4A (pMF1577), Bgl II/Sal I-digested pCMV-Tag4B (pMF1598), or Bgl II/Bam HI-digested pCMV-Tag4B (pMF1599) vectors. To generate pMF214, a bacterial expression plasmid coding for (His)6-HsPex3p(229–364) , the 403 bp Bam HI – Hind III fragment of the PEX3 open reading frame was ligated into Bam HI/Hind III-restricted pQE30. All essential constructs were verified by DNA sequencing (Agowa).
The haploid Saccharomyces cerevisiae strains BY4741 (genotype: MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0; accession number Y00000) and BY4742 (genotype: MATα ; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0; accession number Y10000) as well as the corresponding null mutants (open reading frame::KanMX4) of arf1 (Y03890), arf2 (Y03835), arf3 (Y01870 and Y11870), arl1 (Y03304), cin4 (Y06744), pex5 (Y03603), vps21 (Y01865), ypt6 (Y05171), ypt7 (Y00575), ypt10 (Y03404), ypt11 (Y01140), ypt31 (Y06583), ypt32 (Y04576), ypt52 (Y05085), and ypt53 (Y07232) were obtained from Euroscarf. Yeast cells were transformed and selected as described . In order to construct the double arf1arf3 deletion strain, the single arf1 (Y03890) and arf3 (Y11870) deletion strains were crossed with each other. Diploids were selected on -lys -met medium and checked by mating type PCR. The diploids were sporulated and subjected to tetrad analysis. Double deletion strains were selected on medium containing Geneticin (2:2 segregation for growth on Geneticin indicates that the two mutations are together) and verified by PCR analysis. To assay yeast cells for growth in the presence of oleate as a sole carbon source, the cells were pelleted, washed, and resuspended in sterile water to an optical density of 0.4 at 600 nm. Ten-fold serial dilutions were spotted on oleic acid plates containing 0.1% (v/v) oleic acid, 0.4% (v/v) Tween-40, 0.1% (w/v) yeast extract (Difco), 2% (w/v) agar, and synthetic dropout medium .
The polyclonal antiserum against (His)6-HsPex3p(229–364) was raised in New Zealand White rabbits as previously described . The mouse polyclonal antiserum against bovine catalase and the rabbit polyclonal antisera against HsPex13p, HsPex14p, HsPex19p, rat peroxisomal thiolase, and rat liver peroxisomal matrix proteins (ab-MF16; used to detect catalase and urate oxidase on Western blot) are described elsewhere [63, 64, 68, 69]. The 1-9E10.2 hybridoma cell line producing anti-Myc antibody  was obtained from A.T.C.C. All other primary antibodies were commercially obtained. This list includes (i) rabbit polyclonal antibodies against Arf (Santa Cruz Biotechnology, sc-9063), glutamate dehydrogenase (Rockland Immunochemicals, 100–4158), PMP70 (Zymed Laboratories, 71–8300), HA (GeneTex, GTX29110), cytochrome P450 4A (Affinity BioReagents, PA3-033), and LAMP2 (Sigma, L 0068), (ii) an affinity-purified goat polyclonal antibody against Arf (Santa Cruz Biotechnology, sc-7622), (iii) a rabbit monoclonal antibody against Arf1 (Abcam, ab32524, clone [EP442Y]), and (iv) mouse monoclonal antibodies against Arf3 (BD Biosciences, 610785, clone ), nucleoporin 60 (BD Biosciences, N43620), Bip/GRP78 (BD Biosciences, G73320), pan-cadherin (Sigma, C 1821), Golgi 58 K (Sigma, G 2404), Arf (Abcam, ab2806, clone [1D9]), Arf5 (Abnova, H00003428-M05, clone [1B4]), Arf6 (Santa Cruz Biotechnology, sc-7971, clone [3A-1]), and HA (Roche, 11583816001, clone [12CA5]). The goat anti-rabbit and anti-mouse IgGs conjugated to 12 nm and 18 nm colloidal gold particles, respectively, were obtained from Jackson ImmunoResearch Laboratories. All other secondary antibodies were from Sigma.
Cell culture, transfections, and (immuno)fluorescence microscopy
Primary hepatocytes from mouse embryos (13.5 days) of Arf6+/+ and Arf6-/- littermates were isolated and cultivated as described . After 12 hours, the cells were fixed with ice-cold paraformaldehyde (4% (w/v) in PBS), washed with PBS, and stored until needed. Potorous tridactylis (kangaroo rat) kidney (Ptk2) cells (a gift from Dr. M. Mareel, University Hospital of Ghent, Belgium), allowing for easier imaging given their flat profile , were cultured in DMEM/F-12 medium supplemented with 10% (v/v) fetal calf serum, 2 mM Glutamax (Invitrogen), an antibiotic-antimycotic (100 μg/ml penicillin G, 100 μg/ml streptomycin sulphate, 0.25 μg/ml amphotericin B) mixture (Invitrogen), and 5 μg/ml Plasmocin (Amaxa) in a humidified 37°C, 5% CO2 incubator, and transiently transfected by using Lipofectamine Plus (Invitrogen). The cells were fixed with paraformaldehyde (see above) 36 hours post-transfection. Processing of the cell samples for (immuno)fluorescence microscopy was carried out as described , and fluorescence was evaluated on a CellM imaging station (Olympus) equipped with U-MNUA2, U-MNIBA3, and U-MWIY2 fluorescence mirror units. The Olympus image analysis and particle detection software was used to determine the peroxisome density in Arf6+/+ and Arf6-/- cells.
Preparation of subcellular fractions and isolation of peroxisomal membranes
Rat liver was homogenized in 0.25 M sucrose, 5 mM MOPS (pH 7.2), 1 mM EDTA (pH 7.2), 0.1% (v/v) ethanol, 1 mM dithiothreitol (DTT), and proteinase inhibitor (PI) mixture (1 μg/ml aprotinin, 0.5 μg/ml leupeptin, 1 μg/ml α2-macroglobulin, and 1 μg/ml chymostatin) and fractionated by differential centrifugation into a nuclear, heavy mitochondrial, light mitochondrial, microsomal, and cytosolic fraction as described before . Peroxisomes were purified from the light mitochondrial fraction by (i) isopycnic centrifugation in an iso-osmotic self-generating Percoll gradient , (ii) centrifugation in a Nycodenz step gradient , or (iii) centrifugation of peroxisome-enriched fractions from the Percoll gradient in a Nycodenz step gradient . In order to isolate peroxisomal membranes, peroxisomal peak fractions in the Nycodenz gradient were subjected twice to sonication in 10 mM pyrophosphate buffer (pH 9.0) and centrifuged at 100,000 × g for 1 hour . To increase the purity of the peroxisomal membranes, the membrane pellets were resuspended in 1 ml of sucrose 52% (w/v) in 0.1 M sodium carbonate (pH 11.5) and floated in an alkaline linear sucrose gradient essentially as described previously , with minor modifications. Briefly, resuspended membrane pellets were carefully layered under 4 ml of a continuous gradient (ranging from 12% to 44% (w/v) sucrose in 0.1 M sodium carbonate (pH 11.5)). In order to avoid the pelleting of protein aggregates at the bottom of the centrifugation tubes, a 100 μl cushion of sucrose 80% (w/v) in 0.1 M sodium carbonate was layered under the resuspended membrane pellets. The sucrose gradient was centrifuged at 170,000 × g for 18 hours in a SW55Ti rotor (Beckman Instruments) and fractionated into 200–250 μl aliquots. The proteins present in each fraction were precipitated with trichloroacetic acid and deoxycholate, and processed for SDS-PAGE and immunoblotting.
Percoll, Nycodenz and Percoll/Nycodenz gradient fractions enriched with peroxisomes were fixed in a freshly prepared solution containing 0.25% (v/v) glutaraldehyde-containing, 5 mM MOPS (pH 7.2), 1 mM EDTA (pH 7.2), 1 mM DTT, 0.1% (v/v) ethanol, and PI mixture. In order to prevent osmotic rupture of the organelles, the fixative was supplemented with 0.25 M sucrose (Percoll fractions) or Nycodenz (final density: 1.20 g/ml) (Nycodenz and Percoll/Nycodenz fractions). Fixed samples containing 75 μg or 150 μg of protein were pelleted (11,000 × g, 2 min) and washed by resuspension and pelleting in (i) homogenization buffer (see above), (ii) TBS buffer (0.1 M Tris-HCl (pH 7.4), 9% (w/v) NaCl), and (iii) TBS buffer supplemented with 0.1% (w/v) glycine. Non-specific protein binding sites were blocked for 45 min in TBS buffer supplemented with 1% (w/v) BSA, and the organelle preparations were subsequently incubated with the primary antibodies (mouse 1D9 (1:10 – 1:500) or mouse anti-Arf6 (1:50 – 1:100) and rabbit anti-Pex14p (1:20 – 1:1000) or rabbit anti-PMP70 (1:20 – 1:1000) diluted in TBST (TBS buffer supplemented with 0.05% (v/v) Tween-20)) overnight at 4°C. The next morning, the preparations were washed three times with TBS buffer, incubated with the secondary antibodies (goat anti-rabbit (1:5 – 1:20) and anti-mouse IgGs (1:5 – 1:10) conjugated to 12 nm and 18 nm colloidal gold particles, respectively, diluted in TBST) for two hours at 4°C, and washed three times with TBS buffer (and pelleted for 2 min at decreasing pelleting speeds: 11,000 × g, 5,000 × g, and 2,000 × g). Negative controls were processed in parallel by addition of TBST buffer instead of the primary antibodies. Hereafter, the immuno-treated organelle samples were fixed in TBS buffer supplemented with 0.1 M cacodylate (pH 7.4) and 1% (v/v) glutaraldehyde for 30 min, and subsequently washed with 0.1 M cacodylate (pH 7.4). Afterwards, the samples were post-fixed with aqueous potassium ferricyanide-reduced osmium tetroxide (final concentration: 1% (w/v) OsO4; 150 mg/ml K4 [Fe(CN)6]) for 45 min, shortly rinsed with Milli-Q water, and immobilized on Formvar-coated 400 mesh nickel grids (see below) or embedded in tepid agar-agar (7.5% (w/v) in Milli-Q). The in agar-agar-embedded pellets were carefully cut into small blocks and dehydrated by passage through graded series of ethanol (70% (v/v), 80% (v/v), 90% (v/v), and absolute), transferred to propylene oxide, and embedded in Epoxy resin (Agar 100 Resin Kit, Agar Scientific). The Epon blocks were trimmed and ultrathin sections (75 nm and 300 nm) were put on a 200 mesh copper/rhodium grid. The organelles were contrasted with 2% (w/v) uranyl acetate and Reynold's lead citrate for 2 min and 40 sec, respectively, and rinsed with Milli-Q water. To immobilize immunostained or non-immunostained glutaraldehyde-fixed organelle samples directly on Formvar-coated 400 mesh nickel grids, the grids were first incubated with a poly-L-lysine solution (0.01% (w/v) poly-L-lysine, 0.625% (w/v) boric acid, 0.955% (w/v) sodium tetraborate) for 45 min and subsequently incubated on a drop of the organelle samples for another 45 min. Next, the grids containing immunostained samples were put on a drop of TBS for 10 min, shortly rinsed with the same buffer, and dried. The grids containing non-immunostained organelle samples were washed for 15 min on a drop of TBS and 5 min on a drop of TBS supplemented with 0.1% (w/v) glycine, blocked for 45 min on a drop TBS supplemented with 4% (w/v) BSA, and for one hour incubated with primary and secondary antibodies diluted in TBST supplemented with 0.1% (w/v) BSA. Each antibody incubation was ended by washing the immobilized organelles 12 times for 5 min on a drop of TBS. Negative controls were processed in parallel by addition of TBST buffer instead of the primary antibodies. Finally, the organelle samples were shortly rinsed with TBS, negatively stained by incubating them for 3 min with 0.25% (w/v) uranyl acetate, and dried on a filter paper. All samples were examined with a LEO 906 electron microscope (Zeiss).
Statistical analyses were performed with SAS (Statistical Analysis System) software. ANOVA (Analysis of Variance) was used to determine the differences among independent groups of numerical values, and multiple comparisons with Tukey-Kramer adjustment were used to determine which groups were significantly different from the wild-type group. The significance level was chosen to be 0.05.
We thank Dr. S.R. Terlecky (Wayne State University School of Medicine, Detroit, Michigan) for critically reading the manuscript, Ms. M. Brams (LIPIT, K.U.Leuven, Belgium) and Ms. E. Vanderheyden (Department of Biology, K.U.Leuven) for their excellent technical support, Mr. G. Magdowski, Mr. G. Kripp, and Ms. B. Pfeiffer (all three of the Institute für Anatomie und Zellbiologie II, Justus-Liebig Universität, Germany) for their professional help in the electron microscopic work, and Mr. P.J. Thilakarathne (Leuven Biostatistics and Statistical Bioinformatics Centre, K.U.Leuven, Belgium) for his guidance and suggestions on statistical analyses.
This work was supported by grants from the Flemish Government (Geconcerteerde Onderzoeksacties GOA2004/08), the Fonds voor Wetenschappelijk Onderzoek – Vlaanderen (Onderzoeksproject G.0754.09), the FP6 European Union Project 'Peroxisome' (LSHG-CT-2004-512018), as well as the German Research Foundation (DFG KFO 181/1, P3).
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