Fig. 4

The effect of HIF-1α on LPS-induced epithelial model damage to Caco-2 cells is regulated by the mTOR/P70S6K pathway. (A) Rapamycin (mTOR inhibitor) decreased TEER of Caco-2 cell monolayer, and MHY1487 (mTOR activator) increased TEER of Caco-2 cell monolayer. DMOG (HIF-1α activator) can rescue the decrease of TEER caused by rapamycin, and BAY87-2243 (HIF-1α inhibitor) can rescue the increase of TEER caused by MHY1487; (B) Rapamycin increased the FD4 concentration in Caco-2 cell monolayer, while MHY1487 had opposite effect. DMOG rescued the increase in FD4 concentration caused by rapamycin, and BAY 87-2243 rescued the decrease in FD4 concentration caused by MHY1487; (C) Western blot was used to detect the expression of p-mTOR, mTOR, p-P70S6K, P70S6K, HIF-1α and TJs (ZO-1, occludin and claudin-1)

Con: control group, LPS: LPS group, L + R: LPS + rapamycin group, L + M: LPS + MHY1487 group, L + R + D: LPS + rapamycin + DMOG group. L + M + B: LPS + MHY1487 + BAY 87-2243 group. *P < 0. 05, **P < 0. 01 vs. LPS group; ##P < 0. 01 vs. L + R group; △P < 0. 05, △△P < 0. 01 vs. L + M group