Figure 8From: A thin layer angiogenesis assay: a modified basement matrix assay for assessment of endothelial cell differentiationDirect RNA extraction and RT-qPCR analysis of HUVEC immediately after the TLA assay. Fold relative COX-2 mRNA to GAPDH expression in VEGF-treated HUVEC undergoing differentiation in 24-well plates in the TLA assay. RNA was directly extracted from the cells and targets measured by Taqman RT-qPCR; n = 4 separate experiments.Back to article page