Figure 8

Visualization of autophagic vacuoles with MDC in MCF-7aro cells. MCF-7aro cells were incubated in the absence (A) or in the presence (B) of 25 μM of compound 4a in medium containing 1nM T. After 72 hr incubation with the compounds, cells were treated with MDC for 1 hr at 37°C, washed with PBS and analysed by fluorescence microscopy. The formation of autophagic vacuoles in MCF-7aro cells treated with compound 4a was indicated by punctuated MDC labelling in the cytoplasm.