Figure 9From: Mechanism of nuclear factor of activated T-cells mediated FasL expression in corticosterone -treated mouse Leydig tumor cellsRecruitment of NFAT to the FasL promoter region. ChIP analysis of the FasL promoter region was carried out on cells treated with CORT. The chromatin DNA selected by anti-NFAT antibody was amplified with FasL specific primers. Lane 1, marker; Lane 2, the input of mLTC-1 cells; Lane 3, the ChIP DNA from mLTC-1 cells untreated with CORT. Lane 4, the ChIP DNA from mLTC-1 cells treated with 100 nM CORT; Lane 5, the ChIP DNA from mLTC-1 cells treated with 100 nM CORT and 100 mg/ml CsA; Lane 6, non-specific IgG; Lane7, non-specific primer.Back to article page