Figure 2From: Mechanism of nuclear factor of activated T-cells mediated FasL expression in corticosterone -treated mouse Leydig tumor cellsThe enhancement of FasL mRNA and protein expression in CORT-treated mLTC-1 cells. A. Expression of FasL mRNA was determined by RT-PCR in mLTC-1 cells. Band intensity was normalized to the β-actin product generated using specific primers to β-actin. Lane 1. Marker; Lane 2. The level of FasL mRNA in mLTC-1 cells untreated with CORT; Lane 3. The level of FasL mRNA in mLTC-1 cells treated with 100 nM CORT for 12 h; Lane 4. The level of FasL mRNA in mLTC-1 cells treated with 100 nM CORT plus 100 ng/ml CsA for 12 h. The level of FasL mRNA was increased following the CORT treatment. The mRNA level was decreased in mLTC-1 cells with CORT and CsA treatment. B. Expression of FasL protein in mLTC-1 cells was determined by Western blot. Lane 1, 2, 3 is without CORT treatment, with CORT treatment and CORT plus CsA, respectively. The expression of FasL was increased with CORT treatment and decreased with CORT plus CsA treatment.Back to article page