Table 1 Primers used in the study
S. No. | Clone | Primer No. | Sequence | Restriction sites |
|---|---|---|---|---|
1 | NP-FL | 35104 | 5'-cgcgaattc atggcgaccaaaggc | EcoRI |
35575 | 3'-gaccccgggccc attgtcgtactcctc | ApaI | ||
2 | obpNLS+NC | 36823 | 5'-cgc gaattc atccaaatgtgcaccg | EcoRI |
62638 | 3'-cgcgggccc tttgatcattctgat | ApaI | ||
3 | obpNLS+NTer | 39815 | 5'-gggctcgag catccaaatgtgc | XhoI |
39821 | 3'-gcggaattc gcgccagattcgtct | EcoRI | ||
4 | obpNLS+CTer | 39820 | 5'-gggctcgag caagaaaactggagga | XhoI |
62638 | 3'-cgcgggccc tttgatcattctgat | ApaI | ||
5 | bpNLS-1R | 39815 | 5'-gggctcgag catccaaatgtgc | XhoI |
39813 | 3'-gcgcccggg tcatctcctccactt | ApaI | ||
6 | bpNLS-1M | 39815 | 5'-gggctcgag catccaaatgtgc | XhoI |
39818 | 3'-gcgcccggg tcatctcatccactt | ApaI | ||
7 | bpNLS-2R | 39814 | 5'-gcggaattc aggagagaactcatc | EcoRI |
62638 | 3'-cgcgggccc tttgatcattctgat | ApaI | ||
8 | bpNLS-2M | 39819 | 5'-gcggaattc atgagagaactcatc | EcoRI |
62638 | 3'-cgcgggccc tttgatcattctgat | ApaI | ||
9 | NPΔ obpNLS | 39815 | 5'-gggctcgag catccaaatgtgc | XhoI |
40466 | 3'-gcggaattc aggatctttccc | EcoRI | ||
39817 | 5'-gaggaattc caagctaataatggt | EcoRI | ||
62638 | 3'-cgcgggccctttgatcattctgat | ApaI | ||
10 | NP41–89 | 39815 | 5'-gggctcgag catccaaatgtgc | XhoI |
40466 | 3'-gcggaattc aggatctttccc | EcoRI | ||
11 | NP122–197 | 39817 | 5'-gaggaattc caagctaataatggt | EcoRI |
62638 | 3'-cgcgggccctttgatcattctgat | ApaI | ||
12 | 2bpNLS+NSP6 | 39820 | 5'-gggctcgag caagaaaactggagga | XhoI |
39821 | 3'-gcggaattc gcgccagattcgtct | EcoRI |