Figure 2
From: GxcDD, a putative RacGEF, is involved in Dictyostelium development
The CH domain of GxcDD acts as a membrane anchor. (A) Cells expressing GFP-CH were fixed and stained with actin specific mAb act1-7. GFP-CH and actin co-localized only partially as indicated by arrows. (B) Subcellular fractionation (SCF) and Triton X-100 insoluble fractionations (TIC) of cells expressing GFP-CH were done as described in Materials and methods. Supernatant (S) and pellet (P) fractions were analysed using a GFP monoclonal antibody. Comitin is used as a marker for the membrane fraction and α-actinin as a marker for the cytosolic fraction. GFP-CH was exclusively found in the membrane fractions and did not associate with the cytoskeleton.