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Table 3 Effect of trypsin inhibitors on IL-8 release from A549 cells induced by trypsin

From: Induction of release and up-regulated gene expression of interleukin (IL)-8 in A549 cells by serine proteinases

Compound (μg/ml)

IL-8 released (pg/ml)

 

8 h

16 h

Medium alone

173 ± 54

581 ± 91

SBTI 10

154 ± 47

631 ± 102

SBTI 30

194 ± 52

646 ± 100

α1-AT 10

nd

505 ± 105

Trypsin 0.1

294 ± 78

993 ± 185

Trypsin 0.1 + SBTI 10

176 ± 47*

567 ± 110*

Trypsin 0.1 + SBTI 30

173 ± 49*

590 ± 120*

Trypsin 0.1 + α1-AT 10

nd

556 ± 133*

Trypsin 0.3

718 ± 147

1061 ± 366

Trypsin 0.3 + SBTI 10

158 ± 45*

543 ± 122*

Trypsin 0.3 + SBTI 30

256 ± 67*

654 ± 141*

Trypsin 0.3 + α1-AT 10

nd

579 ± 131*

  1. Data shown are mean ± SE for five separate experiments. Trypsin, inhibitor, and trypsin with inhibitor were added to cells for 8 h or 16 h. * P < 0.05 compared with the corresponding concentration of trypsin alone. α1-AT = α1-antitrypsin, SBTI = soybean trypsin inhibitor, nd = not done.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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