Figure 2From: Thalidomide attenuates nitric oxide mediated angiogenesis by blocking migration of endothelial cellsThalidomide attenuates tube formation in ECs. Trypsinized ECs in suspension culture were seeded on collagen coated 12 well plates with 70%cell density. After 24 hours, the ECs were treated with different concentrations of thalidomide, (50, 100 and 150 μg/ml) and incubated at 37°C/5%CO2 for 12 hours and thereafter, total number of tubes were counted. Number of tubes was reduced in all, 50 100 and 150μg/ml thalidomide treated ECs respectively.Back to article page