Figure 4
From: Regulation of cell cycle by the anaphase spindle midzone
Presence of aurora B in both daughter cells following microsurgery of nocodazole-treated cells. An NRK cell at early anaphase was treated with 10 μM nocodazole for ~10 min before microsurgical cut between segregated chromosomes (cutting site indicated by the dotted line, a). Then the daughter cells were released from nocodazole by washing twice with fresh medium. Immunofluorescence of aurora B showed that both daughter cells contained dot-like structures of aurora B along the cell cortex (c, d, arrows). Bar, 20 μm.