Figure 3
From: Mutational analyses of the signals involved in the subcellular location of DSCR1
Confocal laser scanning microscope images of distinct DSCR1-punctual mutants expression in CHO-1 (line 1, A-D), COS-7 (line 2, E-H) and HEK 293 cells (line 3, I-L). Subcellular location of: DSCR1A160 (A, E, I), DSCR1A166 (B, F, J), DSCR1A160+A166 (C, G, K) and DSCR1A108+A112 (D, H, L). The different constructions obtained by site-directed mutagenesis are included in the section on material and methods and analyzed in detail in the results section. Scale bars are indicated in the figure. Mutated sequences are shown at the bottom (dots indicate the amino acids replaced by alanines).