Figure 4From: Increased protein aggregation in Zucker Diabetic Fatty rat brain: identification of key mechanistic targets and the therapeutic application of hydrogen sulfideThiol groups and CBS (cystathionine beta synthase) expression in ZDF (zucker fatty diabetic rat) and Lean frontal brain. (A) CBS expression is lower in ZDF brains. (B) Real-time PCR analysis of the expression of CBS relative to β-actin, and (C) Thiol group level in ZDF is lower than Lean. Western blot data showing protein of interest (upper part) and β-actin (lower part). Data are Means ± SEM (n ≥ 5 per group), * <0.0001 (difference to control gray bars), magnification 40×; unpaired t-test. Western blot expression is normalized to β-actin; lanes of western blot insets are in the same order as in the X-axis.Back to article page