Figure 3From: Collagen/β1 integrin interaction is required for embryoid body formation during cardiogenesis from murine induced pluripotent stem cellsExpression patterns of collagen and integrins during EB formation. (A) mRNA expression of collagen (Col1A1, Col1A2, Col3A1) and its integrin receptors (Itga1, Itga2, Itgb1) determined by semiquantitative RT-PCR. (B to D) Real-time PCR analysis of Col1A1, Itga1 and Itgb1, normalized to β-actin, in mESCs, miPSCs, and EBs (days 3 and 5). Experiments were performed in triplicate. *Significant at p < 0.05. (E) Immunostaining of collagen type I in miPSC-derived EBs on day 3. Scale bars = 200 μm.Back to article page