Figure 3

PKC-δ inhibitor rottlerin abrogates IFN-α-induced activity of TRAIL promoter in Daudi cells. Daudi cells were transfected with a TRAIL-Luc reporter, followed by incubation with medium alone for 24 h. The cells were pretreated with rottlerin for 1 h, stimulated with 1,000 U/ml IFN-α for 24 h, and then assayed for luciferase activity. Reporter activity was normalized relative to internal control Renilla luciferase values. Normalized data from experimental group are expressed as relative luciferase activity compared with those seen in control group. Each point represents mean ± SD from three independent experiments. *Significantly different (p < 0.05) from control without inhibitor.