Gene | Sequence | Product size | Annealing temp |
---|---|---|---|
sFRP1 | Forward: ATCTCTGTGCCAGCGAGTTT | 202bp | 55°C |
Reverse: AAGTGGTGGCTGAGGTTGTC | |||
sFRP2 | Forward: AGGACAACGACCTTTGCATC | 217bp | 55°C |
Reverse: TTGCTCTTGGTCTCCAGGAT | |||
sFRP3 | Forward: AAACTGTAGAGGGGCAAGCA | 227bp | 55°C |
Reverse: GGCAGCCAGAGCTGGTATAG | |||
sFRP4 | Forward: CGATCGGTGCAAGTGTAAAA | 181bp | 54°C |
Reverse: GACTTGAGTTCGAGGGATGG | |||
sFRP5 | Forward: GATGTGCTCCAGTGACTTTG | 352bp | 60°C |
Reverse: GCAGGGGTAGGAGAACATGA | |||
GAPDH | Forward: CAGAACATCATCCCTGCATCCACT | 185bp | 59°C |
Reverse: GTTGCTGTTGAAGTCACAGGAGAC | |||
L19 | Forward: GGACAGAGTCTTGATGATCTC | 194bp | 51°C |
Reverse: CTGAAGGTCAAAGGGAATGTG | |||
β-actin | Forward: GCACCAAGGATGGAGATGTT | 173bp | 55°C |
 | Reverse: GGACAGAGTCTTGATGATCTC |  |  |