Figure 6

SM22 depletion reduces stress induced ROS production. ROS levels were measured in control REF52 cells (A) or SM22-depleted REF52-depleted cells (B) in normal serum conditions or following serum withdrawal for 24 or 48 h. In control REF52 cells both 24 h or 48 h serum withdrawal resulted in a 15-fold increase in ROS levels. REF52 cells depleted for SM22 (B) however, had 66% lower intrinsic ROS levels, and a considerably reduced ROS response to serum withdrawal for 24 h or 48 h (4-fold and 11-fold increase over untreated respectively). Control cell population is shaded in black, 24 and 48 serum withdrawal populations (24SW and 48SW) are indicated by black or grey lines respectively.