Figure 5

Wnt signaling is downregulated by SOX4-plakoglobin complex. A. Luciferase assay of LNCaP HASOX4 stable cell line co-transfected with 40 ng of TK-Renilla with either 500 ng of TOP-flash or FOP-flash plasmids. Luciferase activity was increased after Wnt inducement, but decreased in combined WNT3A+LMB treatment. (n = 3; error bars represent SEM; *, p < 0.05 for a one-sided paired t-test) B. Quantitative results of ChIP of β-catenin by realtime-PCR. β-catenin promoter occupancies of Wnt downstream targets, AXIN2, c-MYC, and DKK1, were increased with Wnt treatment, but is strongly reduced by combined WNT3A+LMB treatment. (n = 3; error bars represent SEM; *, p < 0.05; **, p < 0.01 for a one-sided paired t-test)