Time after thawing | The devices used for FTC | Untreated cells* |
---|
 | i3C | standard cryo-vial |  |
---|
3 hours
| 4.3% ± 2.1 (3% - 7%) |  | 9.9% ± 6.2 (1% - 19%) |
overnight
| 10.0% ± 10.0 (2% - 20%) | 3.0% ± 2.8 (1% - 5%) | 7.0% ± 4.3 (1.5% - 10%) |
- U937 cells underwent a freezing - thawing cycle as described in Materials and Methods. The percentage of early apoptotic (Annexin V positive and PI negative) cells was tested at appropriate time points after thawing. Values are average ± SD of 2-3 experiments (500-1000 individual cells). The range of results obtained for the different experiments (as percentages) is presented in brackets. *Untreated U937 cells, which did not undergo FTC, were evaluated at the corresponding time points.