Figure 5
From: Heme and non-heme iron transporters in non-polarized and polarized cells
Mutational analysis of the N-terminal cytoplasmic domain of DMT1A-I reveals the crucial signal for localizing to the plasma membrane. A. Summary of the mutational analysis of the N-terminal cytoplasmic domain of DMT1A-I. Amino acid sequences of the N-terminal cytoplasmic domain of wild-type DMT1A-I and its various mutants are displayed together with their localizations. GFP-tagged DMT1A-I L16A (a and d) and mCherry-tagged DMT1A-I WT (b) are transfected into HEp-2 cells. The cells were fixed and stained with antibodies against LAMP2 (e). B. MDCK cells stably expressing GFP-tagged DMT1A-I (a), GFP-tagged DMT1B-I (b), and GFP-tagged DMT1A L16A (c) were grown on Transwell and analyzed after their polarization.