Figure 4From: Pelota interacts with HAX1, EIF3G and SRPX and the resulting protein complexes are associated with the actin cytoskeletonPELO interacts with HAX1, EIF3G and SRPX in vivo. A, Total protein from HeLa cells, which are co-transfected with HA-tagged PELO and either Myc-tagged HAX1, EIF3G or SRPX constructs, were precipitated with monoclonal anti-Myc antibodies. The precipitates of co-transfected cells with PELO-HA:Myc-HAX1 (IP1), PELO-HA:Myc-EIF3G (IP2) and PELO-HA:Myc-SRPX (IP3) were probed in immunoblot analysis with polyclonal anti-HA antibodies. IPC, control co-immunoprecipitation (coIP) assay using anti-mouse IgG serum. B-C, In reciprocal coIP experiments, cells lysates from co-transfected HeLa cells were precipitated with either polyclonal anti-HA (B) or anti-PELO antibodies (C). Precipitated proteins (IP) were detected with anti-Myc antibodies. IPC, control coIP experiments using anti-rabbit IgG serum.Back to article page