From: R-Ras regulates β1-integrin trafficking via effects on membrane ruffling and endocytosis
Stain | % of cells with ruffles | Ruffles per cell (+/- S.D.) |
---|---|---|
Endogenous R-Ras | 42 of 51 (82%) | 2.9 ± 0.5 |
with R-Ras siRNA | 0 of 30 (0%) | N/A |
Cholera Toxin B | 29 of 34 (85%) | 3.3 ± 0.7 |
with R-Ras siRNA | 2 of 15 (9%) | 0.3 ± 0.02 |
GFP-R-Ras(wt) | 150 of 189 (79%) | 3.2 ± 0.6 |
GFP-R-Ras(38V) | 191 of 210 (91%) | 8.9 ± 1.1 |
GFP-R-Ras(41A) | 5 of 202 (2%) | 0.1 ± 0.003 |