Figure 5From: CRK9 contributes to regulation of mitosis and cytokinesis in the procyclic form of Trypanosoma bruceiLocalization of CRK9 in procyclic cells. CRK9 fused to the 3xHA tag was inserted into the pc-BLA-PTP vector. After linearization, transfection into T. brucei, and selection using blasticidin (10 μg/ml), cells expressing CRK9-3HA at the endogenous level were obtained. Transfected and control cells were analyzed by Western blotting for the HA tag as indicated (A). The blot was also probed with anti α-tubulin to verify equal sample loading. Localization of CRK9 was determined by fixing the transfected cells and staining them with a FITC-conjugated anti-HA antibody (B). DAPI was used to visualize the nucleus and kinetoplast and merged images were created using the imageJ software.Back to article page