Figure 2
Cellular distribution of the Tubby-domain and PLCδ1PH domain expressed in HEK293-AT1 cells. The GFP-Tubby-domain and PLCδ1PH-GFP were expressed either separately (panel A) or were co-expressed (here the PLCd1PH was tagged with mRFP, panel B) in HEK293-AT1 cells. Confocal images show a stronger localization of the Tubby domain to the PM. The PM vs. cytoplasmic fluorescent ratios were calculated from line intensity histograms taken across representative sections of the cells (shown by arrows in the Figures on Panel B). Representative intensity histograms are shown in Panel C from cells 1 and 2 as shown in Panel B. The background fluorescence was subtracted from both the average PM and cytoplasmic fluorescence values when calculating the FPM/Fcyto ratios. These values are found in the text.