Figure 10

Inhibition of RhoA GTPase does not prevent OI-V-induced disassembly of epithelial apical junctions. Activity of Rho GTPase in HPAF-II cells was blocked by either incubation with a cell-permeable inhibitor C3 toxin (2 μg/ml for 3 h; A) or overexpression of a dominant-negative N19 RhoA mutant (B), and effect of this inhibition on OI-V induced disassembly of AJ and TJ was determined by immunofluorescence labeling and confocal microscopy. Neither pharmacological inhibitor, nor dominant-negative RhoA mutant prevent disassembly of epithelial junctions induced by the PKC activator. Bar, 20 μm.