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Table 1 Primers employed in RT-PCR

From: Testis-expressed profilins 3 and 4 show distinct functional characteristics and localize in the acroplaxome-manchette complex in spermatids

Primer

Tm

product

Accession-No.

5'-CAGTGAATTC ATGGCCGGT TGGCAGAG-3'

5'-CGATGGATCC AGCAGCTAGAACCCAGAGTC-3'

68°C

71°C

446 bp

ORF

(bp 99 → 526)

huProfilin2a

NM_053024

5'-TTGAATTC ATGAGTGACTGGAAGGGCTACA-3'

5'-TTGGATCC GTTCACGGTTTATTCTGGTCTCC-3'

65°C

68°C

467 bp

ORF

(bp 59 → 524)

mProfilin3 NM_029303

5'-CAGTGAATTC AGCATGAGTCACTTGCG-3'

5'-GATGGATCC TTAGTTTCCCTTTTTCCTTAG-3'

63°C

64°C

405 bp

ORF

(bp 861 → 1250)

mProfilin 4

NM_028376

5'-AGTGAATTC GGGAACATGAGCCATT-3'

5'-GATGGATCC CTCTGATGACTTAACTTCCT-3'

61°C

65°C

406 bp

ORF (bp171 → 557)

huProfilin4

BC029523

5'-TAGAATTC ATGGATGGATGATATCGCCGCGC-3'

5'-AAGGATCC AAGCCATGCCAATCTCA-3'

67°C

70°C

1236 bp

ORF (bp74 → 1282)

hu β-actin, NM_001101

5'-CAGTGAATTC TTTACCGACCACCAAGAAACTCAG-3'

5'-CGATGGATCC TCATATGGCACCCGAAAAGAGC-3'

68°C

71°C

668 bp, FH1 + part. FH2-domain (bp904 → 1551)

huDiaphanous3 NM_030932

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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