Figure 3From: A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serumInhibition of BMP mediated activation of BRE-Luc with specific blocking antibodies. C2C12BRE cells were treated as in Figure 1 with (A) 5 ng/ml BMP4, (B) 20 ng/ml BMP6 and (C) 5 ng/ml BMP9 with or without increasing concentrations of BMP4, BMP6 and BMP9 specific blocking antibodies respectively (D, E, F, G, H, I). C2C12BRE cells were treated as in Figure 1 with 5 ng/ml BMP4 (D), 20 ng/ml BMP6 (E), 2 ng/ml BMP9 (F), 50 ng/ml BMP2 (G), 10 ng/ml BMP7 (H) or 20 ng/ml BMP10 (I) in the presence or absence of 5 ng/ml BMP4 antibody, 2 ng/ml BMP6 antibody or 0.1 ng/ml BMP9 antibody. Luciferase activity was normalised to protein content and fold inductions relative to non-BMP treated samples are shown. Each point represents the mean ± SEM of three independent experiments performed in quadruplicate.Back to article page