Figure 1
From: Loss of Dictyostelium HSPC300 causes a scar-like phenotype and loss of SCAR protein
HSPC300 as part of the SCAR complex. (A) Alignment of HsHSPC300 (Accession number AAF28978), AtHSPC300 (Accession number Q94JY4) and DdHSPC300 (Dictybase reference DDB0231424). (B) Western blots showing SCAR and PIR121 levels in growing cells. Equal quantities of protein were probed with anti-SCAR and PIR121 antibodies. SCAR protein is almost undetectable in IR55 hspc300 null cells. PIR121 levels are essentially normal in IR48 scar null and IR55 hspc300 null cells. (C) Rescue of SCAR levels in IR55 hspc300 null cells expressing Myc-tagged or untagged HSPC300. Total lysates from wild type, hspc300 null and hspc300 null cells expressing either Myc-HSPC300 or an untagged control were blotted and probed with anti-SCAR antibodies. (D) HSPC300 is part of the SCAR complex. Lysates prepared from IR55 hspc300 null cells expressing either Myc-HSPC300 or an untagged control were immunoprecipitated with anti-Myc antibody and probed with anti-SCAR and anti-PIR121 antibodies.