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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Development of an in vitro human alveolar epithelial air-liquid interface model using a small molecule inhibitor cocktail

Fig. 4

Expression of AEC markers in YAC-treated HPAEpiCs and conventional A549 cells under both submerged and ALI culture conditions. Passage 3 cells were used for analysis. (A) Comparative gene expression analysis of A549 cells and each lot of HPAEpiCs using AQP5 as a marker for AECIs, and SFTPA1, SFTPB, SFTPC, and SFTPD as markers for AECIIs. Data expressed as mean ± standard error (n = 3, three independent experiments). Data obtained from each lot of HPAEpiCs were compared with those from A549 cells using one-way ANOVA with Dunnett’s multiple comparison test; *p < 0.05; ND, not detected. (B) Immunocytochemical analysis of ALI-cultured, YAC-treated HPAEpiCs and conventional A549 cells. Blue color indicates nuclei (DAPI), and green color indicates AQP-5/proSP-C (Alexa 488). Scale bar: 50 μm

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BMC Molecular and Cell Biology

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