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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Mechanical stretch leads to increased caveolin-1 content and mineralization potential in extracellular vesicles from vascular smooth muscle cells

Fig. 4

RhoA kinase (ROCK) inhibition increases Cav-1 EVs independently of intracellular Cav-1 levels. Phalloidin staining shows reorientation of F-actin (green) in VSMCs subjected to 10% cyclic strain (A) versus non-stretched VSMCs (B). ROCK inhibitor Y-27632 (10 μM) disrupts F-actin in both stretched (C) and static (D) VSMCs. This associates with increased EV Cav-1 release from stretched (E) and non-stretched (F) VSMCs treated with Y-27632 versus vehicle control. Intracellular Cav-1 levels are unaltered by ROCK inhibition in stretched (G) or static (H) VSMCs. n = 4. Data are mean ± SD. (*P < 0.05). (Scale bar: 10 µm). Data represents mean ± SD. Full-length blots are presented in Supplementary Fig. 4

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BMC Molecular and Cell Biology

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