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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Mechanical stretch leads to increased caveolin-1 content and mineralization potential in extracellular vesicles from vascular smooth muscle cells

Fig. 3

Immunofluorescence staining shows Cav-1 (red) transitioning from alignment along actin filaments in non-stretched VSMCs)(A) to a more dispersed, cytosolic distribution after application of 10% cyclic strain for 72 h (B). C Pre-treatment with the dynamin inhibitor dynasore (40 μM) reduces Cav-1 internalization in VSMCs subjected to 10% strain for 72 h compared to control. D This inhibition of Cav-1 endocytosis by dynasore decreases Cav-1 release in EVs from stretched VSMCs by 2.41-fold (P < 0.01). E Levels of the exosomal marker CD63 are unchanged in EVs from control versus 10% stretched VSMCs. F Knockdown of the Cav-1 gene in cyclically stretched VSMCs significantly decreased Cav-1 protein levels in EVs released from these cells. n = 3. Data are mean ± SD (**P < 0.01) (Scale bar: 2 µm). Data represents mean ± SD. Full-length blots are presented in Supplementary Fig. 3

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BMC Molecular and Cell Biology

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