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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Mechanical stretch leads to increased caveolin-1 content and mineralization potential in extracellular vesicles from vascular smooth muscle cells

Fig. 2

Effect of cyclic strain on caveolin-1 distribution in vascular smooth muscle cells (VSMCs). A Application of 10% cyclic strain for 72 h significantly decreases the intracellular level of caveolin-1 compared to non-stretched control VSMCs, as quantified by Western blotting. B Exposure of VSMCs to 10% cyclic strain for 72 h significantly increases the release of caveolin-1 positive extracellular vesicles (EVs) compared to non-stretched controls, as determined by Western blotting. C Cyclic strain results in increased trafficking of caveolin-1 from VSMCs into extracellular vesicles as compared to static control conditions, based on normalization of extracellular vesicular caveolin-1 content to intracellular caveolin-1 levels in corresponding VSMCs. D Cyclic mechanical strain elevates intracellular cholesterol levels in VSMCs. n = 9 Data are presented as mean ± SD (*P < 0.05, ****P < 0.0001). Data represents mean ± SD. Full-length blots are presented in Supplementary Fig. 2

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BMC Molecular and Cell Biology

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