Fig. 4
From: Optimization of seeding density of OP9 cells to improve hematopoietic differentiation efficiency
Improving the initial seeding number of OP9 cells shortened the whole time of co-culture system without changing the efficiency of hematopoietic differentiation. A Comparison of the expression of hematopoietic-related genes during differentiation between the experimental subgroup (10.4 × 104 cells, 13.5 × 104 cells, and 16.6 × 104 cells/cm2 in a six-well plate) and the control group. Data are presented as means ± SD (n = 3). These results were consistent with flow cytometry. B Cell distribution in CFU from sorted CD34 positive cells for culturing 14–16 days in a Semi-solid medium. E, erythroid; G, granulocyte; GM, granulocyte/macrophage; M, macrophage. C Morphology of different colony-forming unit types, including M, GM, G and E. Scale bar = 100 mm