Fig. 3
From: Optimization of seeding density of OP9 cells to improve hematopoietic differentiation efficiency
Determination of differentiation efficiency in the hESCs/OP9 co-culture system. A The expression of CD34 in undifferentiated human embryonic stem cells (HN14) and OP9 cells, separately. B Percentage of CD34+ cells by flow cytometry (x-axis) in the control group. The peak of hematopoietic differentiation on D12. C Percentage of CD34+ cells by flow cytometry (x-axis) in the experimental group. a to f show six different seeding numbers in 6-well plates, 3.1 × 104 cells, 5.2 × 104 cells, 7.3 × 104 cells, 10.4 × 104 cells, 13.5 × 104 cells, and 16.6 × 104 cells/cm2, respectively. Single-cell suspension from hESCs/OP9 co-culture obtained at the indicated time points was labeled with CD34 (x-axis). The number indicates the percentage of positive cells in the corresponding group. Except for (a) group, the peak of hematopoietic differentiation on D10. D Evaluation of the expression of hematopoietic-related genes on day 10 in the experimental group. RNA was extracted, and gene expression was quantified by qRT-PCR. Statistical results of phase-specific genes are shown as the mean ± SD, n = 3. ## P < 0.01 and # P < 0.05 compared with the 16.6 × 104 cells/cm2 group. ▲▲ P < 0.01 and ▲ P < 0.05 compared with the 13.5 × 104 cells/cm2 group. ★★ P < 0.01 and ★ P < 0.05 compared with the 10.4 × 104 cells/cm2 group