Fig. 2
From: Optimization of seeding density of OP9 cells to improve hematopoietic differentiation efficiency
The morphology of ES cells on OP9 stromal cells during differentiation on days 8, 10 and 12. (A) a to f show the morphological changes of HN14 cells on six different seeding numbers of OP9 cells, i.e. 3.1 × 104 cells, 5.2 × 104 cells, 7.3 × 104 cells, 10.4 × 104 cells, 13.5 × 104 cells, and 16.6 × 104 cells/cm2, respectively, on days 8, 10 or 12 (bar = 100 μm). B The morphologies of differentiated HN14 colonies during co-culture on days 8, 10 and 12 in the control group (bar = 100 μm). In all groups, the presence of rounded cells at the edge of the colonies