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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Simple, low-cost, and well-performing method, the outgrowth technique, for the isolation of cells from nasal polyps

Fig. 6

Tissue is on the petri dish placed and 1 ml PBS is added to keep the tissue from becoming dry A. The tissue is cut into small pieces of approx. 1–1.5 mm with surgical scissors. Small pieces were placed on the 12-well culture plate (2 pieces/well) and stand under the safety cabinet without lid for 5–10 min to dry and stick on the surface of the culture dish. Approx. 350 μl of BEGM was then given carefully without mobilizing tissue pieces from the dish (B)

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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