Fig. 2From: Simple, low-cost, and well-performing method, the outgrowth technique, for the isolation of cells from nasal polypsCumulative number of tissue pieces from which cells grew out. Tissues from three patients were taken for isolation of epithelial cells. 24 small-cut pieces of tissue per sample from each patient (two pieces per well in a 12-well plate) were used for outgrowth of epithelial cells. The cumulative number of tissue samples from which cells grew out was assessed every 2 days. Cells grew out from almost all tissue pieces (67/72) within 8 days – 19 of 24 tissue pieces from Polyp 1 yielded cells. Epithelial cells grew out from all tissue pieces of Polyp 2 and Polyp 3Back to article page