Fig. 9

Moesin’s phosphorylation state feeds back to the phosphorylation state of NMII’s regulatory light chain. A-D TIRF images S2R + treated with (A) control RNAi or (B-D) Moesin 3’UTR RNAi and cells fixed and stained for phosphomyosin (left panel, red in merge). C and D Cells were also expressing EGFP-tagged (C) Moesin T559A (middle panel, cyan in merge) or (D) Moesin T559E (middle panel cyan in merge). Scale bar 10 µm. E and F Quantification of the mean (± SEM) (C) Fluorescence and (D) Coalescence Index of cells following RNAi treatment with control (yellow circles) and Moesin 3’UTR (mint green circles) RNAi. We also expressed EGFP-tagged Moesin T559A (plum circles) and Moesin T559E (brown circles) following Moesin 3’UTR RNAi. E Expression of Moesin T559E following Moesin 3’UTR RNAi led to a statistically significant decrease in the amount of phosphorylated NMII regulatory light chain (**p-value = 0.004123,***p-value = 0.00057, one-way ANOVA with Tukey’s post-hoc analysis, N = 3). Similarly, expression of Moesin T559E following Moesin 3’UTR RNAi led to a decrease in Coalescence (*p-value = 0.0149, **p-value = 0.00495,***p-value = 0.00566, one-way ANOVA with Tukey’s post-hoc analysis, N = 3)