Fig. 7

Flw regulates cellular contractility through its interaction with Moesin. A-D Phase-contrast images of S2R + cells following perfusion of Fog and treatment with (A) control, (B) Flw, (C) Moesin, and (D) Flw and Moesin RNAi. Yellow arrowheads denote contracted cells and cyan arrowheads denote rounded cells. Scale bar 10 µm. E and F Quantification of the fraction (± SEM) of (E) Contracted cells and (F) Rounded cells, treated with control (yellow circles), Moesin (mint green circles), Flw (blue circles), and Flw and Moesin (magenta circles) RNAi. E Depletion of Moesin led to a statistically significant increase in the fraction of contracted cells, while double depletion of Moesin and Flw only led to a partial rescue (**p-value = 0.002304,***p-value = 0.000258, ****p-value < 0.0001, one-way ANOVA with Tukey’s post-hoc analysis, N = 3). F Conversely, depletion of Moesin did not affect the fraction rounded cells we observed, while depletion of Flw led to a statistically significant increase. The double depletion of Moesin and Flw failed to rescue this cell-rounding phenotype ( ****p-value < 0.0001, one-way ANOVA with Tukey’s post-hoc analysis, N = 3)