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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: From network analysis to experimental validation: identification of regulators of non-muscle myosin II contractility using the folded-gastrulation signaling pathway

Fig. 5

PP1 complex members have distinct localization patterns. A-C S2R + cells co-expressing TagRFP-tagged (A) Flapwing (left panel, cyan in merge), (B) MBS (left panel, cyan in merge), or (C) MYPT-75D (left panel, cyan in merge) with SQH-EGFP (middle panel, red in merge) imaged by TIRF microscopy. Yellow lines indicate a representative region of interest where line scans were taken and graphically represented in D-F. Scale bar 10 µm. D-F Line scans from cells co-expressing TagRFP-tagged (D) Flw, (E) MBS, and (F) MYPT-75D (all in cyan), with EGFP-Sqh (red). Graphs represent the Normalized Fluorescent Intensity of 7–10 cells per condition

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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