Fig. 3

RNAi depletion of Oya disrupts the localization of phosphorylated non-muscle myosin II. A-C Drosophila S2R + cells stained for actin (right panel, cyan in merged image) and phosphomyosin (middle panel, red in merged image) treated with (A) control, (B) Oya, or (C) Sqh RNAi. Yellow arrowheads denote coalesced phosphomyosin contractile network while cyan arrowheads denote a dispersed network. Scale bar is 10 µm. D The quantification of the ratio of the fluorescent intensities of phoshomyosin to actin following RNAi treatments. The mean (± SEM) phosphomyosin:actin ratio in Oya depleted cells (magenta circles) was statistically significantly lower than that of control treated cells (yellow circles) but greater than that of Sqh depleted cells (blue circles) (**** p < 0.0001, one-way ANOVA with Tukey’s post-hoc analysis). (E) Quantification of the coalescence index measuring the degree of phosphomyosin coalescence in cells treated with control (yellow circles), Oya (magenta circles), and Sqh RNAi (blue circles). The mean (± SEM) coalescence in Oya depleted cells is less than control treated cells yet higher than Sqh depleted cells (** p-value = 0.00295, **** p-value < 0.0001, one-way ANOVA with Tukey’s post-hoc analysis N = 3)