Fig. 2

Computationally identified candidates lead to the inhibition of NMII contractility. A-H Phase-contrast images of S2R + cells treated with control (A and B), CG10374 (C and D), Flapwing (E and F), or Oya (G and H) RNAi. Cells were either perfused with control cell media (A, C, E, G) or treated with Fog-conditioned media (B, D, F, H); yellow arrows indicate cells that have contracted following Fog perfusion. The white box in (A) denotes uncontracted cells shown at higher magnification while the yellow box in (B) denotes the contracted cells shown at higher magnification (right). Scale bars 10 µm. I-K Scatter plots quantifying the fraction of contracted cells with and without Fog perfusion following RNAi treatments with CG10274 RNAi (I), Flapwing RNAi (J), and Oya RNAi (K). I There was no statistically significant difference between control and CG10274 RNAi cells with and without Fog treatment, however in there was an inhibition of cellular contractility following RNAi treatment with Flapwing and Oya as compared to control RNAi treated cells (****p-values < 0.0001, Student's T-test, N = 3). (L & M) Results from RT-qPCR indicating the efficacy of RNAi treatments for cells treated with Flapwing RNAi (L) and Oya (M). There was a statistically significant reduction in Flw mRNA as compared to control treatments (****p-value > 0.0001, Student’s t-test, N = 3), similarly, we observed a statistically significant reduction in Oya mRNA following RNAi treatment as compared to controls (** p-value = 0.0024, ****p-value > 0.0001, one-way ANOVA, N = 5)