Table 3 The system of PCR reaction
From: Glycyrrhizin inhibits LPS-induced inflammatory responses in goat ruminal epithelial cells in vitro
Reagents | Addition |
|---|---|
SYBRGreen Mix | 12.5 µL |
Upstream primer F | 0.5 µL |
Downstream primer R | 0.5 µL |
ddH2O | 9.5 µL |
cDNA templet | 2 µL |
Total volume | 25 µL |