Table 2 The system of reverse transcription reaction
From: Glycyrrhizin inhibits LPS-induced inflammatory responses in goat ruminal epithelial cells in vitro
Reagents | Addition |
|---|---|
RNA-Primer Mix | 12 µL |
5×RT Reaction Buffer | 5 µL |
25 mM dNTPs | 1 µL |
25 U/µL RNase Inhibitor | 1 µL |
200 U/µL M-MLV Rtase | 1 µL |
Oligo(dt)18 | 1 µL |
ddH2O (DNase-free) | 4 µL |
Total volume | 25 µL |