Table 1 The primer sequence of targeted genes
From: Glycyrrhizin inhibits LPS-induced inflammatory responses in goat ruminal epithelial cells in vitro
Gene | Primer sequences (5’-3’) | Length (bp) |
|---|---|---|
NF-κB(p65) (XM-018043384.1) | F: GTGTAAAGAAGCGGGACTTGG R: GCGGTTGTCAAAGATGGGATG | 203 |
TNF-α (NM-001286442.1) | F: CCACGTTGTAGCCAACATCAG R: AGATGAGGTAAAGCCCGTCAG | 134 |
IL-1β (XM-013967700.2 | F: CCACCTCCTCTCACAGGAAATG R: GATACCCAAGGCCACAGGAATC | 100 |
IL-6 (NM-001285640.1) | F: TACCTGGACTTCCTCCAGAAC R: CGAATAGCTCTCAGGCTGAAC | 245 |
IL-8 (XM-005681749.3) | F: TCTTGGCCGCTTTCCTGCTC R: TTTCGCAGTGTGGCCCACTC | 162 |
IL-12 (NM-001285700.1) | F: GACCAAACCTCAGCCAAAG R: GACACAGATGCCCATTCAC | 104 |
GAPDH (XM-005680968.3) | F: GCCCTGAGGCTCTCTTCCA | 101 |
R: GCGGATGTCGACGTCACA |