Fig. 1
From: BMP9 maintains the phenotype of HTR-8/Svneo trophoblast cells by activating the SDF1/CXCR4 pathway
BMP9 promoted proliferation, migration and invasion of HTR-8/SVneo cells. After transfection of pcDNA3.1-BMP9 and si-BMP9 plasmids for 24 h, the following indexes were measured. (A) The expression of of BMP9 in HTR-8/SVneo cells. (B) The proliferation of HTR- 8/SVneo cells was evaluated by CCK8 assay. (C) The migratory capacity of cells was determined by wound healing assay. (D) The invasive ability of cells was detected by transwell assay. (E) Western blot was used to detect the expression levels of migration and invasion-related proteins (MMP-2, MMP-9, E-cadherin and N-cadherin). (F) The expression of MMP-2 and MMP-9 in HTR-8/SVneo cells. (G) The expression of E-cadherin and N-cadherin in HTR-8/SVneo cells. * means P < 0.05 compared to ov-NC, # means P < 0.05 compared to si-NC.