Fig. 5

ELF4 deficiency aggravated inflammation and pyroptosis. A In kidney tissues of WT and ELF4^−/− mice, IL-6, TNF-α, IL-18, and IL-1β protein levels were detected by way of western blot. B GSDMD, N-GSDMD, and caspase-11 protein levels were surveyed in kidney tissues of WT and ELF4^−/− mice using western blot. The levels of inflammatory cytokines (IL-6, TNF-α, IL-18, and IL-1β; C), and pyroptosis-related proteins (GSDMD, N-GSDMD, and caspase-11; D) were detected using western blot in HK-2 cells. Mouse n = 5. Cell n = 3. * p < 0.05 WT sham or control group, # p < 0.05 ELF4^−/− sham, WT I/R, or OGD/R group